College of Biosystems Engineering and Food Science, Zhejiang Univ., Hangzhou, 310058, Zhejiang, China.
Zhejiang Key Lab. for Agro-Food Processing, Zhejiang R&D Center for Food Technology and Equipment, Hangzhou, 310058, Zhejiang, China.
J Food Sci. 2019 Jan;84(1):111-120. doi: 10.1111/1750-3841.14405. Epub 2018 Dec 12.
Although different delivery systems have been developed to overcome the limits of Epigallocatechin-3-gallate (EGCG) and quercetin in food application, none have referred to their simultaneous encapsulation. In this study, these two polyphenols were successfully co-loaded into liposomes. Under the optimal conditions (lecithin-total polyphenols ratio 25:1, lecithin-cholesterol ratio 6:1, lecithin-Tween 80 ratio 8:1 and ultrasonic time 2 min), the mean size, polydispersity index (PDI) and zeta potential of liposomes were 111.10 ± 0.52 nm, 0.259 ± 0.006 and -19.83 ± 0.45 mV, with an encapsulation efficiency of 64.05 ± 1.56% and 61.73 ± 2.55% for EGCG and quercetin, respectively. After 30-day storage, an increase of 4.05% was observed in the mean size with no significant change (P ≥ 0.05) in the PDI and zeta potential. Moreover, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay revealed a synergistic antioxidant effect of the two compounds in liposomal system. These results demonstrated that co-encapsulation of EGCG and quercetin enhances their effectiveness. PRACTICAL APPLICATION: EGCG and quercetin are natural polyphenols abound in the human diet with diverse biological activities. These two polyphenols were successfully co-encapsulated into a homogeneous and stable liposomal system. Interestingly, a synergistic antioxidant effect of the two polyphenols was observed due to co-encapsulation. This indicated that the simultaneous delivery of EGCG and quercetin was an attractive approach to improve their functionality for expanding their application in food, cosmetic and pharmaceutical industries.
尽管已经开发出不同的输送系统来克服表没食子儿茶素没食子酸酯（EGCG）和槲皮素在食品应用中的局限性，但没有一种方法涉及到它们的同时包封。在这项研究中，这两种多酚被成功地共同负载到脂质体中。在最佳条件下（卵磷脂-总多酚比 25:1、卵磷脂-胆固醇比 6:1、卵磷脂-Tween 80 比 8:1 和超声时间 2 分钟），脂质体的平均粒径、多分散指数（PDI）和 Zeta 电位分别为 111.10±0.52nm、0.259±0.006 和-19.83±0.45mV，EGCG 和槲皮素的包封效率分别为 64.05±1.56%和 61.73±2.55%。在 30 天的储存期后，平均粒径增加了 4.05%，而 PDI 和 Zeta 电位没有显著变化（P≥0.05）。此外，2,2-二苯基-1-苦基肼（DPPH）测定法显示，两种化合物在脂质体系统中的协同抗氧化作用。这些结果表明，EGCG 和槲皮素的共同包封增强了它们的功效。实际应用：EGCG 和槲皮素是天然多酚，广泛存在于人类饮食中，具有多种生物活性。这两种多酚被成功地共同包封到均匀稳定的脂质体系统中。有趣的是，由于共同包封，观察到了两种多酚的协同抗氧化作用。这表明同时输送 EGCG 和槲皮素是一种有吸引力的方法，可以提高它们的功能，扩大它们在食品、化妆品和制药行业的应用。
