Bez''iazychnaia T S, Moskvichev B V
Prikl Biokhim Mikrobiol. 1988 Jul-Aug;24(4):481-3.
Low-molecular modification of proteolytic enzymes with aldehydes and anhydrides of carboxylic acids as well as with 2,4,6-trinitrobenzene sulphonic acid was studied. Specific activities of the enzymes were found to be dependent on the modification degree of their amino groups. The retaining of high activities in the region of low extents of enzyme modification enabled biocatalysts with activities similar to those of the native enzymes to be prepared.
研究了用羧酸的醛类和酸酐以及2,4,6-三硝基苯磺酸对蛋白水解酶进行低分子修饰。发现酶的比活性取决于其氨基的修饰程度。在酶修饰程度较低的区域保持高活性，使得能够制备出活性与天然酶相似的生物催化剂。
