Gershkovich A A, Kibirev V K
Bioorg Khim. 1988 Nov;14(11):1461-88.
The review describes approaches to designing chromogenic and fluorogenic substrates for proteolytic enzymes, mainly for assay of serine proteinases. Principles of substrate polypeptide chain construction and some methods for detection of chromogenic and fluorogenic products of their hydrolysis are considered. The use of these substrates for the study of blood clotting enzymes and for clinical diagnostics is briefly treated. Methodology of chemical synthesis of principal chromogenic and fluorogenic substrates is also discussed.
这篇综述描述了用于蛋白水解酶，主要是丝氨酸蛋白酶检测的生色和荧光底物的设计方法。考虑了底物多肽链构建的原则以及检测其水解生色和荧光产物的一些方法。简要介绍了这些底物在血液凝固酶研究和临床诊断中的应用。还讨论了主要生色和荧光底物的化学合成方法。
