S. A. Richardson, Dr. A. M. Eissa, Department of Chemistry, University of Warwick, Coventry, CV4 7AL, UK.
T. M. Rawlings, Dr. J. Muter, Prof. J. J. Brosens, Division of Biomedical Sciences, Reproductive Health Unit, Clinical Science Research Laboratories, Warwick Medical School, University of Warwick and Tommy's National Centre for Miscarriage Research, University Hospitals Coventry and Warwickshire NHS Trust, Coventry, CV2 2DX, UK.
Macromol Biosci. 2019 Feb;19(2):e1800351. doi: 10.1002/mabi.201800351. Epub 2018 Dec 13.
A novel strategy for the surface functionalization of emulsion-templated highly porous (polyHIPE) materials as well as its application to in vitro 3D cell culture is presented. A heterobifunctional linker that consists of an amine-reactive N-hydroxysuccinimide ester and a photoactivatable nitrophenyl azide, N-sulfosuccinimidyl-6-(4'-azido-2'-nitrophenylamino)hexanoate (sulfo-SANPAH), is utilized to functionalize polyHIPE surfaces. The ability to conjugate a range of compounds (6-aminofluorescein, heptafluorobutylamine, poly(ethylene glycol) bis-amine, and fibronectin) to the polyHIPE surface is demonstrated using fluorescence imaging, FTIR spectroscopy, and X-ray photoelectron spectroscopy. Compared to other existing surface functionalization methods for polyHIPE materials, this approach is facile, efficient, versatile, and benign. It can also be used to attach biomolecules to polyHIPE surfaces including cell adhesion-promoting extracellular matrix proteins. Cell culture experiments demonstrated that the fibronectin-conjugated polyHIPE scaffolds improve the adhesion and function of primary human endometrial stromal cells. It is believed that this approach can be employed to produce the next generation of polyHIPE scaffolds with tailored surface functionality, enhancing their application in 3D cell culture and tissue engineering whilst broadening the scope of applications to a wider range of cell types.
提出了一种用于乳液模板化高多孔（聚 HIPE）材料表面功能化的新策略，以及将其应用于体外 3D 细胞培养的方法。利用一种由胺反应性 N-羟基琥珀酰亚胺酯和光活化的硝基苯叠氮基组成的杂双功能连接体，N-琥珀酰亚胺基-6-（4'-叠氮-2'-硝基苯氨基）己酸酯（磺基-SANPAH），对聚 HIPE 表面进行功能化。通过荧光成像、傅里叶变换红外光谱和 X 射线光电子能谱，证明了该方法能够将一系列化合物（6-氨基荧光素、全氟丁基胺、聚乙二醇双胺和纤连蛋白）连接到聚 HIPE 表面。与其他现有的聚 HIPE 材料表面功能化方法相比，该方法简便、高效、多功能且温和。它还可以用于将生物分子附着到包括促进细胞黏附的细胞外基质蛋白在内的聚 HIPE 表面。细胞培养实验表明，纤连蛋白缀合的聚 HIPE 支架可改善原代人子宫内膜基质细胞的黏附和功能。据信，这种方法可用于制备具有定制表面功能的新一代聚 HIPE 支架，从而增强其在 3D 细胞培养和组织工程中的应用，并将应用范围扩大到更广泛的细胞类型。
