School of Environment, Harbin Institute of Technology, Harbin 150090, China.
School of Environment, Harbin Institute of Technology, Harbin 150090, China.
J Hazard Mater. 2019 Mar 15;366:395-401. doi: 10.1016/j.jhazmat.2018.12.002. Epub 2018 Dec 3.
The performance of the lysozyme catalysis on excess sludge (ES) hydrolysis and decomposition was investigated in this study. For this purpose, the release of soluble organic matters from sludge flocs, extracellular polymeric substances (EPS) changes in composition and distribution and the quantity variations of microorganisms were monitored. Results indicated that lysozyme boosted the ES hydrolysis significantly with approximately 236.5 mg/L soluble chemical oxygen demand (SCOD), 58.6 mg/L polysaccharide and 662.7 mg/L protein release within 240 min at the lysozyme dosage of 150 mg/gSS. Arising lysozyme dosages (from 0 to 150 mg/gSS step by step) could dramatically enhance the efficiency of the enzyme on ES with the concentration of polysaccharide increased from 84.6 mg/L to 143.2 mg/L and protein increased from 325.0 mg/L to 987.7 mg/L in total EPS. The decomposition effect of lysozyme on microorganisms improved with dosage, about 15.4%, 17.5% and 20.2% bacteria and 56.3%, 57.2% and 65.0% archaea were disintegrated at the lysozyme dosages of 50, 100 and 150 mg/gSS, respectively. However, fungi were barely influenced by the enzymatic catalysis. Tryptophan-protein like substances and aromatic protein were the dominant ES lysis compositions in EPS.
本研究考察了溶菌酶对剩余污泥（ES）水解和分解的催化性能。为此，监测了污泥絮体中可溶性有机物的释放、细胞外聚合物物质（EPS）组成和分布的变化以及微生物数量的变化。结果表明，在溶菌酶用量为 150mg/gSS 时，ES 的水解在 240 分钟内显著提高，释放出约 236.5mg/L 的可生化需氧量（SCOD）、58.6mg/L 的多糖和 662.7mg/L 的蛋白质。随着溶菌酶用量（从 0 增加到 150mg/gSS）的增加，酶对 ES 的效率显著提高，多糖浓度从 84.6mg/L 增加到 143.2mg/L，总 EPS 中的蛋白质从 325.0mg/L 增加到 987.7mg/L。溶菌酶对微生物的分解效果随着剂量的增加而提高，在溶菌酶剂量为 50、100 和 150mg/gSS 时，细菌分别减少了约 15.4%、17.5%和 20.2%，古菌减少了 56.3%、57.2%和 65.0%。然而，真菌几乎不受酶催化的影响。色氨酸-蛋白质样物质和芳香族蛋白质是 EPS 中 ES 裂解的主要成分。
