School of Pharmaceutical Sciences (Shenzhen), Sun Yat-sen University, Guangzhou, 510006, PR China.
School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, PR China.
Biomed Pharmacother. 2019 Jan;109:1361-1371. doi: 10.1016/j.biopha.2018.11.013. Epub 2018 Nov 12.
Zengye decoction (ZYD), a traditional Chinese medicinal formula, has been used in the treatment of various chronic diseases, such as constipation and skin dryness syndrome. Clinically, the specific mechanisms and targets of ZYD for treating disease remain unclear. The present study was undertaken to investigate the effects of ZYD on the gut microbiota and host metabolites in aged constipated rats and the relationship between the intestinal microbiota and the host. Rats were divided randomly into three groups, the control group (n = 10), recovery group (n = 10) and ZYD group (n = 10). First, the aged constipation model was established for the ZYD group and recovery group. Then, rats in the ZYD group were treated with ZYD. Urinary and faecal samples of each animal were collected in microcentrifuge tubes. Next, 16s rRNA gene sequencing was employed to analyse the composition of the gut microbiome in faecal samples and afterwards the metabolic function of the altered gut microbiota was predicted. Additionally, H NMR profiling was used to detect the alterations of host metabolites in urine and faecal samples to verify the metabolic function results obtained from sequencing. As a result, ZYD reduced the level of harmful bacteria, such as Desulfovibrio, Ruminococcus, Prevotella and Dorea, and increased the abundance of Oxalobacter, Clostridium and Roseburia. The functional prediction of changes in the gut microbiota induced by ZYD revealed that ZYD promoted energy storage, regulated amino acid metabolism, inhibited methane metabolism, strengthened the physiological function of glutathione and reduced bacterial toxin. The H NMR profiles revealed that ZYD regulated the carbohydrates, short chain fatty acids, amino acids and amines in the aged constipated rats. In addition, most metabolic changes observed were related to the function of intestinal microbiota. These results suggest that ZYD can regulate the intestinal microbiota of constipated rats to normal levels and change the endogenous metabolites of the host through the intestinal microbiota to achieve therapeutic effects.
增液汤（ZYD）是一种传统的中药方剂，已用于治疗各种慢性疾病，如便秘和皮肤干燥症。临床上，ZYD 治疗疾病的具体机制和靶点尚不清楚。本研究旨在探讨 ZYD 对老年便秘大鼠肠道微生物群和宿主代谢物的影响，以及肠道微生物群与宿主之间的关系。大鼠随机分为三组，对照组（n=10）、恢复组（n=10）和 ZYD 组（n=10）。首先，为 ZYD 组和恢复组建立老年便秘模型。然后，用 ZYD 治疗 ZYD 组大鼠。收集每只动物的尿液和粪便样本于微量离心管中。接下来，采用 16s rRNA 基因测序分析粪便样本中肠道微生物群的组成，然后预测改变肠道微生物群的代谢功能。此外，采用 1H NMR 谱分析检测尿液和粪便样本中宿主代谢物的变化，以验证从测序获得的代谢功能结果。结果显示，ZYD 降低了有害细菌的水平，如脱硫弧菌、瘤胃球菌、普雷沃氏菌和多雷亚菌，增加了 Oxalobacter、Clostridium 和 Roseburia 的丰度。ZYD 诱导的肠道微生物群变化的功能预测表明，ZYD 促进了能量储存，调节了氨基酸代谢，抑制了甲烷代谢，增强了谷胱甘肽的生理功能，减少了细菌毒素。1H NMR 谱分析表明，ZYD 调节了老年便秘大鼠的碳水化合物、短链脂肪酸、氨基酸和胺类。此外，观察到的大多数代谢变化与肠道微生物群的功能有关。这些结果表明，ZYD 可以调节便秘大鼠的肠道微生物群至正常水平，并通过肠道微生物群改变宿主的内源性代谢物，从而达到治疗效果。
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