Department of Gastroenterology, The Second People's Hospital affiliated to Fujian University of Traditional Chinese Medicine, Fuzhou, 353003, China.
School of Basic Medical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Microb Cell Fact. 2020 Nov 18;19(1):212. doi: 10.1186/s12934-020-01473-3.
In this study, the self-extracted constipation treatment of traditional Chinese medicine extracts was applied to constipated rats. To explore the mechanism and role of the Chinese medicine for the treatment of constipation, the 16S rRNA sequencing and qRT-PCR technology were used to analyze the intestinal flora. We found that the relative abundance of Firmicutes with constipation was significantly higher accounted for 86.7%, while the gut microbiota was significantly changed after taking a certain dose of Chinese medicine, greatly increased the relative abundance of Lactobacillus accounted for 23.1%, enhanced the symbiotic relationships of Lactobacillus with other intestinal flora. The total copies of intestinal bacteria in the constipated rats decreased after taking the traditional Chinese medicine. Finally, this study results provides a theoretical basis for the treatment and understand the mechanism and effect of traditional Chinese medicine on rate constipation.
本研究应用自提取的中药提取物对便秘大鼠进行治疗，以探究中药治疗便秘的机制和作用，采用 16S rRNA 测序和 qRT-PCR 技术分析肠道菌群。我们发现便秘大鼠的厚壁菌门相对丰度显著较高，占 86.7%，而在服用一定剂量的中药后，肠道菌群发生显著变化，显著增加了占 23.1%的乳酸菌相对丰度，增强了乳酸菌与其他肠道菌群的共生关系。便秘大鼠服用中药后肠道细菌总数减少。最后，本研究结果为治疗和理解中药对大鼠便秘的作用机制和效果提供了理论依据。
