Department of Breast Surgery, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, PR China.
Department of Anesthesiology, China-Japan Union Hospital of Jilin University, Changchun, Jilin 130033, PR China.
Biomed Pharmacother. 2019 Jan;109:2128-2135. doi: 10.1016/j.biopha.2018.09.029. Epub 2018 Nov 27.
Altered expression of microRNAs (miRNAs) was involved in prostate cancer progression. However, how miRNAs contributed to prostate cancer development remained unknown. Here, we reported that miR-139-5p levels were decreased in prostate cancer tumor tissues and prostate cancer cell lines. Transfection of miR-139-5p mimics reduced cell proliferation and migration ability of prostate cancer cells. Western blotting and RT-qPCR showed that elevation of miR-139-5p greatly inhibited SOX5 expression in prostate cancer cells. Through regulation of SOX5, enhanced expression of miR-139-5p downregulated TWIST, decreased N-cadherin and Vimentin expression, suggesting inhibition of epithelial-mesenchymal transition (EMT) process. The dual luciferase assay validated that SOX5 was a direct target of miR-139-5p. Additionally, a significant negative correlation between SOX5 mRNA levels and miR-139-5p levels were detected in prostate cancer tumor tissues. Our study indicated that miR-139-5p functioned as a tumor suppressor in prostate cancer cells by regulation of SOX5, and it might be a promising target for prostate cancer patients.
miRNAs（微小 RNA）的表达改变与前列腺癌的进展有关。然而，miRNAs 如何促进前列腺癌的发展尚不清楚。在这里，我们报告 miR-139-5p 的水平在前列腺癌肿瘤组织和前列腺癌细胞系中降低。miR-139-5p 模拟物的转染降低了前列腺癌细胞的增殖和迁移能力。Western blot 和 RT-qPCR 显示，miR-139-5p 的上调极大地抑制了前列腺癌细胞中 SOX5 的表达。通过对 SOX5 的调节，增强表达的 miR-139-5p 下调 TWIST，降低 N-钙黏蛋白和波形蛋白的表达，提示上皮-间充质转化（EMT）过程受到抑制。双荧光素酶报告基因实验验证了 SOX5 是 miR-139-5p 的直接靶基因。此外，在前列腺癌肿瘤组织中检测到 SOX5 mRNA 水平与 miR-139-5p 水平之间存在显著的负相关。我们的研究表明，miR-139-5p 通过调节 SOX5 在前列腺癌细胞中发挥肿瘤抑制作用，它可能是前列腺癌患者有前途的治疗靶点。
Biomed Pharmacother. 2018-11-27
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