Laboratorio de Neurofarmacología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, Xalapa, Veracruz, Mexico.
Hospital General de Zona con Medicina Familiar No. 28, Delegación Veracruz Norte, Instituto Mexicano del Seguro Social (H.G.Z. c/mf. No. 28, Delegación Veracruz Norte, IMSS), Martínez de la Torre, Veracruz, Mexico.
Biomed Pharmacother. 2019 Jan;109:2387-2395. doi: 10.1016/j.biopha.2018.11.111. Epub 2018 Nov 30.
The present study investigated the effects of the flavonoid chrysin (5,7-dihydroxyflavone) on anxiety-like behavior in rats in a model of surgical menopause and evaluated the participation of γ-aminobutyric acid-A (GABA) receptors in these actions. At 12 weeks post-ovariectomy, the effects of different doses of chrysin (0.5, 1, 2, and 4 mg/kg) were evaluated in the elevated plus maze, light/dark test, and locomotor activity test, and comparisons were made with the clinically effective anxiolytic diazepam. The participation of GABA receptors in the actions of chrysin was explored by pretreating the rats with the noncompetitive GABA chloride ion channel antagonist picrotoxin (1 mg/kg). The results showed that chrysin (2 and 4 mg/kg) reduced anxiety-like behavior in both the elevated plus maze and light/dark test, and these effects were similar to diazepam. Pretreatment with picrotoxin had no effects on its own but prevented the anxiolytic-like effects of chrysin in both tests. Chrysin also increased rearing and grooming, without significantly altering the number of crossings in the locomotor activity test; these effects were also similar to diazepam. In conclusion, the flavonoid chrysin produced anxiolytic-like effects through actions on GABA receptors in a model of surgical menopause in rats. These findings support the hypothesis that this flavonoid could be a future natural alternative for ameliorating symptoms of anxiety after surgical menopause in women.
本研究探讨了黄酮类化合物白杨素（5,7-二羟黄酮）对手术去卵巢大鼠模型中焦虑样行为的影响，并评估了γ-氨基丁酸-A（GABA）受体在这些作用中的参与。在卵巢切除术后 12 周，评估了不同剂量白杨素（0.5、1、2 和 4mg/kg）在高架十字迷宫、明暗测试和运动活性测试中的作用，并与临床有效抗焦虑药地西泮进行了比较。通过用非竞争性 GABA 氯离子通道拮抗剂印防己毒素（1mg/kg）预处理大鼠，探讨了 GABA 受体在白杨素作用中的参与。结果表明，白杨素（2 和 4mg/kg）减少了高架十字迷宫和明暗测试中的焦虑样行为，其作用与地西泮相似。印防己毒素预处理本身没有作用，但阻止了白杨素在这两种测试中的抗焦虑作用。白杨素还增加了竖立和梳理行为，而在运动活性测试中，穿越次数没有明显改变；这些作用也与地西泮相似。总之，黄酮类化合物白杨素通过作用于 GABA 受体在大鼠手术去卵巢模型中产生抗焦虑样作用。这些发现支持了这样一种假设，即这种黄酮类化合物可能成为未来改善女性手术后绝经焦虑症状的天然替代品。
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