Laboratorio de Neuropsicofarmacología, Dirección de Neurociencias, Instituto Nacional de Psiquiatría "Ramón de la Fuente Muñíz", Calzada México Xochimilco 101, Col San Lorenzo Huipulco, Delegación Tlalpan, Ciudad de México, Mexico.
Departamento de Farmacobiología, CINVESTAV-Sede Sur. Calzada de los Tenorios 235, Col Granjas Coapa, Delegación Tlalpan, Ciudad de México, Mexico.
J Ethnopharmacol. 2019 Sep 15;241:112006. doi: 10.1016/j.jep.2019.112006. Epub 2019 May 30.
Montanoa tomentosa Cerv. (MT) is a native plant from Mexico used in traditional medicine as a remedy for reproductive impairments and relaxing effects. In previous studies, it has been shown that the endocrine state could modify the antianxiety-like actions of anxiolytic compounds. Although women are the primary user of MT, no studies have evaluated the potential impact of the endocrine milieu on its anti-anxiety actions.
Ascertain the antianxiety effects of M. tomentosa in rats with different hormonal conditions, and to analyze the participation of the GABA receptor in ovariectomized rats treated with MT.
The animal model of anxiety used was the elevated plus-maze (EPM). Rats' endocrine conditions were: a) Low hormone levels (rats in diestrus I and II phases); b) High hormone levels (proestrus/estrus phases); c) No hormones (ovariectomized rats); and d) Rats under progesterone withdrawal (PW). To evaluate the participation of the GABA receptor in the anxiolytic-like action of MT the antagonist picrotoxin was used.
Results showed that MT induced dose-dependent anxiolytic-like actions in rats with low hormone level conditions. Also, MT reduced anxiety-like behavior in female rats under PW, in contrast to diazepam which was ineffective. MT's anxiolytic-like effect was blocked by picrotoxin, suggesting the participation of the GABA receptor complex. However, increased anxiety-like behavior was observed in rats with a high hormone level condition and low doses of MT.
Beneficial anxiolytic-like actions of MT are observed under low hormone conditions, particularly in the PW challenge (a condition that can be related to a premenstrual period). Furthermore, the participation of the GABA receptor is evidenced. However, hormonal variations could induce the opposite effects, hence women should be cautious.
Montanoa tomentosa Cerv.（MT）是一种原产于墨西哥的植物，在传统医学中被用作治疗生殖障碍和放松的药物。在之前的研究中，已经表明内分泌状态可以改变抗焦虑化合物的抗焦虑作用。尽管女性是 MT 的主要使用者，但没有研究评估内分泌环境对其抗焦虑作用的潜在影响。
确定 MT 在具有不同激素条件的大鼠中的抗焦虑作用，并分析 GABA 受体在接受 MT 治疗的去卵巢大鼠中的参与情况。
使用高架十字迷宫（EPM）作为焦虑动物模型。大鼠的内分泌状况为：a）低激素水平（动情前期 I 和 II 期的大鼠）；b）高激素水平（动情前期/动情期的大鼠）；c）无激素（去卵巢大鼠）；d）孕激素撤退（PW）的大鼠。为了评估 GABA 受体在 MT 的抗焦虑样作用中的参与，使用了拮抗剂菽螺环酮。
结果表明，MT 在低激素水平条件下诱导了剂量依赖性的抗焦虑样作用。此外，MT 减少了 PW 下雌性大鼠的焦虑样行为，而地西泮则无效。MT 的抗焦虑样作用被菽螺环酮阻断，表明 GABA 受体复合物的参与。然而，在高激素水平条件和 MT 低剂量下观察到增加的焦虑样行为。
MT 在低激素条件下表现出有益的抗焦虑样作用，特别是在 PW 挑战下（这种情况可能与经前期有关）。此外，证据表明 GABA 受体的参与。然而，激素变化可能会引起相反的效果，因此女性应该小心。
