Laboratorio de Neurofarmacología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Facultad de Química Farmacéutica Biológica, Universidad Veracruzana, Xalapa, Veracruz, Mexico.
Laboratorio de Neurofarmacología, Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, Mexico; Cátedras CONACyT-Instituto de Neuroetología, Universidad Veracruzana, Xalapa, Veracruz, Mexico.
Behav Brain Res. 2021 Jan 15;397:112952. doi: 10.1016/j.bbr.2020.112952. Epub 2020 Oct 2.
Low concentrations of ovarian hormones, among other factors, are associated with greater vulnerability to negative effects of environmental stressors and may trigger anxiety symptoms in females. The flavonoid chrysin (5,7-dihydroxyflavone) exerts anxiolytic-like effects in male and ovariectomized female rats, but it is unknown if chrysin could reduce anxiety-like behavior that naturally occurs through the ovarian cycle phases. The present study evaluated the effect of chrysin on anxiety-like behavior associated with the ovarian cycle phases in rats and the participation of γ-aminobutyric acid-A (GABA) receptors in these actions. The acute effects of chrysin (2 mg/kg) were investigated in female cycling Wistar rats in the elevated plus maze, locomotor activity test, and light/dark test. Diazepam (2 mg/kg) was used as reference anxiolytic drug. The participation of GABA receptor in the anxiolytic actions of chrysin was explored by pretreating the rats with the noncompetitive GABA chloride ion channel antagonist picrotoxin (1 mg/kg). Chrysin and diazepam prevented anxiety-like behavior that was associated with the metestrus-diestrus phase in both the elevated plus maze and light/dark test, and these effects were reversed by picrotoxin, with no significant changes in spontaneous locomotor activity. No significant motor effects of chrysin were detected in either behavioral test during proestrus-estrus or metestrus-diestrus phases, whereas diazepam produced motor hypoactivity in the locomotor activity test during proestrus-estrus phase. These results indicate that the flavonoid chrysin prevents anxiety-like behavior that naturally occurs during metestrus-diestrus in two unconditioned models that are used to evaluate anxiety-like behavior, and these effects were mediated by actions on GABA receptors.
低浓度的卵巢激素等因素与对环境应激源的负面影响更易感性有关，并且可能在女性中引发焦虑症状。类黄酮白杨素（5,7-二羟黄酮）在雄性和去卵巢雌性大鼠中表现出抗焦虑样作用，但尚不清楚白杨素是否可以减轻自然发生在卵巢周期阶段的焦虑样行为。本研究评估了白杨素对大鼠卵巢周期阶段相关焦虑样行为的影响，以及γ-氨基丁酸-A（GABA）受体在这些作用中的参与。在高架十字迷宫、运动活性测试和明暗测试中，研究了白杨素（2 mg/kg）对雌性循环 Wistar 大鼠的急性作用。地西泮（2 mg/kg）用作参考抗焦虑药物。用非竞争性 GABA 氯离子通道拮抗剂 picrotocxin（1 mg/kg）预处理大鼠，探讨了 GABA 受体在白杨素抗焦虑作用中的参与。白杨素和地西泮可预防与发情期-发情后期相关的焦虑样行为，在高架十字迷宫和明暗测试中均如此，这些作用被 picrotocxin 逆转，自发运动活性无明显变化。在发情前期-发情期或发情前期-发情后期期间，在两个用于评估焦虑样行为的非条件模型中，白杨素均未显示出明显的运动效应，而地西泮在发情前期-发情期期间的运动活性测试中产生运动活性降低。这些结果表明，类黄酮白杨素可预防两种非条件模型中自然发生的发情后期-发情后期焦虑样行为，并且这些作用是通过 GABA 受体的作用介导的。
