Minota S, Winfield J B
Division of Rheumatology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599.
Rheumatol Int. 1988;8(4):165-70. doi: 10.1007/BF00270455.
Shedding of cell-surface antigens that react with anti-lymphocyte autoantibodies in systemic lupus erythematosus (SLE) is well-recognized, but the nature of such molecules is unknown. The present investigation demonstrates the rapid shedding of three IgG antibody target molecules of Mr 55,000, 37,000, and approximately 32,000 from the surface of mitogen-activated peripheral T cells during brief incubation at 37 degrees C. Sera lacking IgG anti-lymphocyte antibodies stained none of the three antigens. Absorption of antibody-positive sera with viable HSB-2 cells, a primitive T-cell line lacking HLA antigens and many CD antigens characteristic of mature peripheral T cells, eliminated staining of the shed molecules. These data delineate the number and estimated molecular mass of anti-lymphocyte autoantibody target molecules that are shed from the surface of T cells, and provide further insight into potential mechanisms by which anti-lymphocyte antibodies contribute to the pathogenesis of SLE and related disorders.
系统性红斑狼疮（SLE）中与抗淋巴细胞自身抗体发生反应的细胞表面抗原的脱落已得到充分认识，但此类分子的性质尚不清楚。本研究表明，在37℃短暂孵育期间，有三种分子量分别为55,000、37,000和约32,000的IgG抗体靶分子会从丝裂原激活的外周T细胞表面快速脱落。缺乏IgG抗淋巴细胞抗体的血清对这三种抗原均无染色。用缺乏HLA抗原以及许多成熟外周T细胞特征性CD抗原的原始T细胞系活HSB-2细胞吸收抗体阳性血清后，消除了脱落分子的染色。这些数据明确了从T细胞表面脱落的抗淋巴细胞自身抗体靶分子的数量和估计分子量，并进一步深入了解抗淋巴细胞抗体促成SLE及相关疾病发病机制的潜在机制。
