从人麻风皮肤组织中分离、纯化和定量酚糖脂-1
Isolation, purification and quantification of phenolic glycolipid-1 from human leprosy skin tissues.
作者信息
Venkatesan K, Singh H, Bharadwaj V P, Ramu G
机构信息
Central Falma Institute for Leprosy and RBS College, Agra, India.
出版信息
Trans R Soc Trop Med Hyg. 1988;82(2):321-3. doi: 10.1016/0035-9203(88)90461-0.
Phenolic glycolipid-I, a marker lipid of Mycobacterium leprae, was isolated from skin biopsies obtained from untreated lepromatous leprosy patients by silicic acid and florisil column chromatography and purified by thin layer chromatography. Tissues with varying bacillary loads were analysed for their phenolic glycolipid content. A good correlation was observed between the bacillary population of the tissues and the phenolic glycolipid content.
酚糖脂-I是麻风分枝杆菌的一种标志性脂质，通过硅酸和弗罗里硅土柱色谱法从未经治疗的瘤型麻风患者的皮肤活检组织中分离得到，并通过薄层色谱法进行纯化。对具有不同菌量的组织进行酚糖脂含量分析。观察到组织中的细菌数量与酚糖脂含量之间存在良好的相关性。
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