Wang Shan, Peng Mei Chen, Chen Xun, Yu Liu Chang, Chen Ya, Dan Liu Xiang, Bao Zhou Ri
College of Pharmacy, Hunan University of Chinese Medicine, Changsha, People's Republic of
J Genet. 2018 Dec;97(5):1281-1288.
(), a floral meristem identity gene controls the flowering time and floral transition, and plays an important role in inflorescence and floral organ development. The full-length cDNA for was obtained by rapid amplification ofthe cDNA ends (RACE) so that the roles of in (Lm-AP1) could be better understood. (accession number in GenBank: MF418642) consisted of a 729-bp open reading frame encoding a protein that contained 242 amino acids, had a deduced molecular mass of 27.9919 kDa and a theoretical isoelectric point of 8.75. No signal peptide or transmembranedomains were detected in the sequences located in the nucleus, but it contained conserved sequences for MADS and the K-box. In the secondary structure, the alpha helix accounts for 60.74%, the beta turn 3.72%. The real-time polymerase chain reaction revealed that was more highly expressed in flowers, especially at the fourth flowering stage, which implied that it may play a role in flower development. Other organs, such as stems and leaves, also expressed . This research provided the basis for further analysis of the functional mechanism during development.
（），一个花分生组织特性基因控制开花时间和花的转变，并在花序和花器官发育中起重要作用。通过cDNA末端快速扩增（RACE）获得了（）的全长cDNA，以便更好地理解（）在（Lm-AP1）中的作用。（GenBank登录号：MF418642）由一个729bp的开放阅读框组成，编码一个包含242个氨基酸的蛋白质，推导的分子量为27.9919kDa，理论等电点为8.75。在位于细胞核的序列中未检测到信号肽或跨膜结构域，但它包含MADS和K-box的保守序列。在二级结构中，α螺旋占60.74%，β转角占3.72%。实时聚合酶链反应显示，（）在花中表达量更高，尤其是在第四个开花阶段，这表明它可能在花发育中起作用。其他器官，如茎和叶，也表达（）。本研究为进一步分析（）在（）发育过程中的功能机制提供了依据。
