Long YuQing, Zeng Juan, Liu XiaoRong, Wang ZhiHui, Tong QiaoZhen, Zhou RiBao, Liu XiangDan
College of Pharmacy, Hunan University of Chinese Medicine, Changsha, 410208 Hunan Province China.
Key Laboratory of Germplasm Resources and Standardized Planting of Hunan Large-Scale Genuine Medicinal Materials, Changsha, 410208 Hunan Province China.
3 Biotech. 2024 Jul;14(7):174. doi: 10.1007/s13205-024-04019-1. Epub 2024 Jun 5.
Due to the medicinal importance of the flowers of Xianglei type (XL) , it is important to understand the molecular mechanisms that underlie their development. In this study, we elucidated the transcriptomic and metabolomic mechanisms that underlie the flower development mechanism of two varieties. In this study, 3435 common differentially expressed unigenes (DEGs) and 1138 metabolites were identified. These common DEGs were mainly enriched in plant hormone signal transduction pathways. Metabolomic analysis showed that amino acids were the main metabolites of differential accumulation in wild-type (WT) , whereas in XL, they were flavonoids and phenylalanine metabolites. Genes and transcription factors (TFs), such as MYB340, histone deacetylase 1 (), small auxin-up RNA 32 (), auxin response factor 6 (), PIN-LIKES 7 (), and WRKY6, likely drive metabolite accumulation. Plant hormone signals, especially auxin signals, and various TFs induce downstream flower organ recognition genes, resulting in a differentiation of the two varieties in terms of their developmental trajectories. In addition, photoperiodic, autonomous, and plant hormone pathways jointly regulated the corolla opening. , response regulator 9 (), Gibberellin receptor (), and Constans-like 10 () were closely related to the unfolding of the corolla. These findings offer valuable understanding of the flower growth process of and the excellent XL phenotypes at the molecular level.
The online version contains supplementary material available at 10.1007/s13205-024-04019-1.
由于蕾型（XL）花朵具有药用价值，了解其发育的分子机制很重要。在本研究中，我们阐明了两个品种花朵发育机制的转录组学和代谢组学机制。在本研究中，鉴定出3435个共同差异表达的单基因（DEGs）和1138种代谢物。这些共同的DEGs主要富集在植物激素信号转导途径中。代谢组学分析表明，氨基酸是野生型（WT）中差异积累的主要代谢物，而在XL中，它们是黄酮类化合物和苯丙氨酸代谢物。MYB340、组蛋白去乙酰化酶1（）、小生长素上调RNA 32（）、生长素响应因子6（）、类PIN 7（）和WRKY6等基因和转录因子（TFs）可能驱动代谢物积累。植物激素信号，尤其是生长素信号，以及各种TFs诱导下游花器官识别基因，导致两个品种在发育轨迹上出现分化。此外，光周期、自主和植物激素途径共同调节花冠开放。响应调节因子9（）、赤霉素受体（）和类CONSTANS 10（）与花冠展开密切相关。这些发现为[品种名称]的花朵生长过程以及XL优良表型在分子水平上提供了有价值的理解。
在线版本包含可在10.1007/s13205-024-04019-1获取的补充材料。
