Chen Bingjin, Ge Yajuan, Wang Honglin, Zhu Haihong, Xu Jinyu, Wu Zhenghong, Tang Siye
Department of Obstetrics and Gynecology, Shanghai Fifth People's Hospital of Fudan University, Shanghai 200240, People's Republic of China.
J Genet. 2018 Dec;97(5):1289-1294.
Gestational diabetes mellitus (GDM) represents a common carbohydrate metabolism disorder during pregnancy. The objective of this study was to evaluate the expression levels of mitofusin 2 (MFN2) expression in placentae of GDM patients compared to that in the placental tissues from normal uncomplicated pregnancies. A total of 70 subjects were enrolled from September 2014 to June 2016, including 42 patients with GDM (the GDM group) and 28 normal uncomplicated pregnancies (the control group). Immunohistochemical staining and qRT-PCR were used for the detection of the expression levels and distribution of MFN2 in the placentae of GDM patients and normal controls. Kolmogorov-Smirnov test was used for statistical analysis. < 0.05 and P < 0.01 were used for assessing statistical significance. The baseline characteristics were comparable in both groups. The 1-h and 2-h postprandial glucose levels (PPG) were 7.94±1.26 versus 6.88±0.51 mmol/L and 7.01±1.34 versus 6.14±0.63 mmol/L, respectively,for the GDM group and the control group ( < 0.05). The relative expression levels of MFN2 mRNA were 0.982±1.242 for GDM and 1.257 ± 0.815 for control, respectively, with significant between group difference ( < 0.01). Immunohistochemical staining analysis showed that MFN2 was mostly distributed in the cytoplasm of syncytiotrophoblasts under optical microscopy. Additionally,about 50% of samples of the GDM group were within the intensity of moderate staining of MFN2 and more than 50% of patients in the control group were within the intensity of strong staining of MFN2. The expression levels of MFN2 in GDM placentae was significantly lower compared to that of placentae from normal uncomplicated pregnancies.
妊娠期糖尿病（GDM）是孕期常见的碳水化合物代谢紊乱疾病。本研究的目的是评估GDM患者胎盘组织中线粒体融合蛋白2（MFN2）的表达水平，并与正常未合并症妊娠的胎盘组织进行比较。2014年9月至2016年6月共纳入70名受试者，其中42例GDM患者（GDM组）和28例正常未合并症妊娠者（对照组）。采用免疫组织化学染色和qRT-PCR检测GDM患者和正常对照胎盘组织中MFN2的表达水平及分布。采用Kolmogorov-Smirnov检验进行统计分析。P<0.05和P<0.01用于评估统计学意义。两组的基线特征具有可比性。GDM组和对照组的餐后1小时和2小时血糖水平（PPG）分别为7.94±1.26与6.88±0.51 mmol/L和7.01±1.34与6.14±0.63 mmol/L（P<0.05）。GDM组和对照组MFN2 mRNA的相对表达水平分别为0.982±1.242和1.257±0.815，组间差异有统计学意义（P<0.01）。免疫组织化学染色分析显示，在光学显微镜下，MFN2主要分布在合体滋养层细胞的细胞质中。此外，GDM组约50%的样本MFN2染色强度为中度，而对照组超过50%的患者MFN2染色强度为强阳性。与正常未合并症妊娠的胎盘相比，GDM胎盘组织中MFN2的表达水平显著降低。
