Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, 410128, China; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province, Changde, Hunan, 415000, China.
Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, 410128, China.
Fish Shellfish Immunol. 2019 Mar;86:794-804. doi: 10.1016/j.fsi.2018.12.024. Epub 2018 Dec 14.
The grass carp reovirus (GCRV) has been shown to cause lethal infections in the grass carp Ctenopharyngodon idella (C. idella). In order to investigate the immune response to GCRV infection, the full-length cDNA sequences of coagulation factor VIII (CiFVIII) and plasminogen (CiPLG) from C. idella were cloned and their involvement in the immune response was studied. The immunity factor levels in C. idella with different GCRV resistances were also analyzed. The full-length 2478 bp cDNA of CiFVIII contained an open reading frame of 1965 bp and encoded a putative polypeptide of 654 amino acid residues. The full-length 2907 bp cDNA of CiPLG contained an open reading frame of 2133 bp and encoded a putative polypeptide of 710 amino acid residues. CiFVIII was closely clustered with that of Clupea harengus. CiPLG was first clustered with those of Cyprinus carpio and Danio rerio. CiFVIII transcripts were most abundant in the liver and least in the skin. The highest expression level of CiPLG was observed in liver and the lowest in muscle. Expression levels of CiFVIII in gill, head kidney and spleen, and expression levels of CiPLG in gill, intestine and liver all reached the maximum at 72 h post GCRV infection. In spleen, expression levels of CiFVIII and CiPLG were significantly positively correlated. The activities of T-AOC, LSZ and IgM in R♂ were significantly higher than those in O♂. Likewise, T-AOC and LSZ activities in F1 were significantly higher than f1 individuals (P < 0.01). These results indicated that CiFVIII and CiPLG may play important roles in the immune response to GCRV infection. In addition, antioxidant ability and serum immune factor activity may confer a different viral resistance to C. idella.
草鱼出血病病毒（GCRV）已被证实可引起草鱼（Ctenopharyngodon idella）的致命感染。为了研究草鱼对 GCRV 感染的免疫反应，本研究克隆了草鱼凝血因子 VIII（CiFVIII）和纤溶酶原（CiPLG）的全长 cDNA 序列，并研究了它们在免疫反应中的作用。还分析了不同 GCRV 抗性草鱼的免疫因子水平。CiFVIII 的全长 2478bp cDNA 包含一个 1965bp 的开放阅读框，编码一个 654 个氨基酸残基的假定多肽。CiPLG 的全长 2907bp cDNA 包含一个 2133bp 的开放阅读框，编码一个 710 个氨基酸残基的假定多肽。CiFVIII 与 Clupea harengus 的亲缘关系最为密切。CiPLG 与 Cyprinus carpio 和 Danio rerio 的亲缘关系最为密切。CiFVIII 转录本在肝脏中最丰富，在皮肤中最少。CiPLG 的最高表达水平出现在肝脏，最低表达水平出现在肌肉。在鳃、头肾和脾脏中 CiFVIII 的表达水平，以及在鳃、肠和肝脏中 CiPLG 的表达水平，在 GCRV 感染后 72 小时均达到最大值。在脾脏中，CiFVIII 和 CiPLG 的表达水平呈显著正相关。R♂中的 T-AOC、LSZ 和 IgM 活性显著高于 O♂。同样，F1 中的 T-AOC 和 LSZ 活性也显著高于 f1 个体（P<0.01）。这些结果表明，CiFVIII 和 CiPLG 可能在草鱼对 GCRV 感染的免疫反应中发挥重要作用。此外，抗氧化能力和血清免疫因子活性可能赋予草鱼不同的抗病毒能力。
