College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China; Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization, Hunan Agricultural University, Changsha, Hunan, 410128, China.
Fish Shellfish Immunol. 2024 Aug;151:109712. doi: 10.1016/j.fsi.2024.109712. Epub 2024 Jun 18.
The grass carp (Ctenopharyngodon idella) constitutes a significant economic resource within the aquaculture sector of our nation, yet it has been chronically afflicted by the Grass Carp Reovirus (GCRV) disease. The complement system, a vital component of fish's innate immunity, plays a crucial role in combating viral infections. This research investigates the potential role of MASP1, a key molecule in the lectin pathway of the complement system, in the GCRV infection in grass carp. An analysis of the molecular characteristics of MASP1 in grass carp revealed that its identity and similarity percentages range from 35.10 to 91.00 % and 35.30-91.00 %, respectively, in comparison to other species. Phylogenetically, MASP1 in C. idella aligns closely with species such as Danio rerio, Cyprinus carpio, and Carassius carassius, exhibiting chromosomal collinearity with the zebrafish. Subsequent tissue analysis in both healthy and GCRV-infected grass carp indicated that MASP1's basal expression was predominantly in the liver. Post-GCRV infection, MASP1 expression in various tissues exhibited temporal variations: peaking in the liver on day 5, spleen on day 7, and kidney on day 14. Furthermore, employing Complement Component 3 (C3) as a benchmark for complement system activation, it was observed that MASP1 could activate and cleave C3 to C3b. MASP1 also demonstrated an inhibitory effect on GCRV replication (compared with the control group, VP2 and VP7 decreased by 6.82-fold and 4.37-fold) and enhanced the expression of antiviral genes, namely IRF3, IRF7 and IFN1 (compared with the control group, increased 2.25-fold, 45.38-fold and 22.37-fold, respectively). In vivo protein injection experiments substantiated MASP1's influence on the relative mRNA expression levels of C3 in various tissues and its protein expression in serum. This study also verified that C3 could modulate the expression of antiviral genes such as IFN1 and IRF3.
草鱼（Ctenopharyngodon idella）是我国水产养殖业的重要经济资源，但长期以来一直受到草鱼呼肠孤病毒（GCRV）病的困扰。补体系统是鱼类先天免疫的重要组成部分，在抵抗病毒感染方面发挥着关键作用。本研究探讨了补体系统凝集素途径中的关键分子甘露聚糖结合凝集素 1 相关丝氨酸蛋白酶 1（MASP1）在草鱼 GCRV 感染中的潜在作用。对草鱼 MASP1 的分子特征进行分析，发现其与其他物种的身份和相似性百分比分别为 35.10%至 91.00%和 35.30%至 91.00%。系统进化分析表明，草鱼 MASP1 与斑马鱼、鲤鱼和鲫鱼等鱼类密切相关，与斑马鱼具有染色体共线性。随后对健康草鱼和 GCRV 感染草鱼的组织分析表明，MASP1 的基础表达主要在肝脏中。感染 GCRV 后，不同组织中 MASP1 的表达呈现时间变化：第 5 天肝脏中表达量最高，第 7 天脾脏中表达量最高，第 14 天肾脏中表达量最高。此外，以补体成分 3（C3）作为补体系统激活的基准，观察到 MASP1 可以激活并将 C3 切割为 C3b。MASP1 还表现出对 GCRV 复制的抑制作用（与对照组相比，VP2 和 VP7 降低了 6.82 倍和 4.37 倍），并增强了抗病毒基因的表达，即干扰素调节因子 3（IRF3）、干扰素调节因子 7（IRF7）和干扰素 1（IFN1）（与对照组相比，分别增加了 2.25 倍、45.38 倍和 22.37 倍）。体内蛋白注射实验证实了 MASP1 对不同组织中 C3 的相对 mRNA 表达水平和血清中 C3 蛋白表达的影响。本研究还验证了 C3 可以调节抗病毒基因如 IFN1 和 IRF3 的表达。
