Benske J, Schünke M, Tillmann B
Institute of Anatomy, Christian-Albrechts University, Kiel 1, Federal Republic of Germany.
Acta Orthop Scand. 1988 Oct;59(5):536-41. doi: 10.3109/17453678809148779.
We used polychrome sequential labeling to study the dynamics of subchrondral bone sclerosis during developing arthrosis in knee joints of male STR/1N mice. This technique, using four different colored vital markers, gives detailed information about the site and time of new bone formation in the subchondral bone. In arthrotic joints, we found fluorescent bands arranged excentrically around the marrow cavities always pointing towards the cartilage lesions. The linear separation between the first label and the anatomic surface of the bone marrow cavity varied considerably between the experimental groups. In arthrotic joints, we found bone growth rates 3-4 times greater than in control joints. We also found that the degenerative process in cartilage and subchondral bone was a local phenomenon, because in areas with normal cartilage next to the sclerotic lesions appositional bone growth rates were unaffected.
我们使用多色序列标记法来研究雄性STR/1N小鼠膝关节发育性关节炎过程中软骨下骨硬化的动态变化。该技术使用四种不同颜色的活性标记物，可提供有关软骨下骨新骨形成的部位和时间的详细信息。在关节炎关节中，我们发现荧光带围绕骨髓腔呈偏心排列，且总是指向软骨损伤处。在各实验组中，第一个标记物与骨髓腔解剖表面之间的线性距离差异很大。在关节炎关节中，我们发现骨生长速率比对照关节高3 - 4倍。我们还发现软骨和软骨下骨的退变过程是一种局部现象，因为在硬化病变旁软骨正常的区域，贴附性骨生长速率未受影响。
