Benske J, Schünke M, Tillmann B
Anatomisches Institut der Universität zu Kiel.
Z Orthop Ihre Grenzgeb. 1989 Sep-Oct;127(5):616-9. doi: 10.1055/s-2008-1040301.
Polychrome sequential labeling is used to study the dynamic of subchondral bone sclerosing during developing osteoarthrotic cartilage lesions in knee joints of male STR/1N-mice. The applied technique gives detailed information about site and time of new bone formation in the subchondral tissue using four different colored vital markers. Whereas control animals show regular bone deposition with a circumferential, concentric arrangement of fluorochromes along the various trabeculae, STR/1N-mice with osteoarthrotic cartilage lesions displayed fluorescence bands arranged eccentrically around the marrow cavities always pointing towards the cartilage lesions. The results also demonstrate, that the linear separation between the first label and the anatomic surface of the bone marrow cavity varies considerably in the individual experimental groups. Compared to control knee joints, which show appositional bone growth rates of 25 to 50 microns per 70 days, osteoarthrotic mice reveal increased bone growth rates during developing osteoarthrosis. In early osteoarthrotic cartilage lesions distances between the individual bands are distinctly larger in comparison to distances in advanced lesions. In both cases the rate of appositional bone growth exceeds bone formation in control animals 3 to 4 times.
多色顺序标记法用于研究雄性STR/1N小鼠膝关节骨关节炎软骨损伤发展过程中软骨下骨硬化的动态变化。所应用的技术使用四种不同颜色的活体标记物，提供了软骨下组织新骨形成的部位和时间的详细信息。对照动物显示正常的骨沉积，荧光染料沿不同小梁呈圆周状、同心排列，而患有骨关节炎软骨损伤的STR/1N小鼠则显示荧光带围绕骨髓腔偏心排列，且总是指向软骨损伤部位。结果还表明，在各个实验组中，第一个标记物与骨髓腔解剖表面之间的线性距离差异很大。与每70天骨生长率为25至50微米的对照膝关节相比，患骨关节炎的小鼠在骨关节炎发展过程中显示出更高的骨生长率。在早期骨关节炎软骨损伤中，各个带之间的距离明显大于晚期损伤中的距离。在这两种情况下，骨生长率均超过对照动物的骨形成率3至4倍。
