Ohtani H, Sasano N
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Acta Pathol Jpn. 1988 Jul;38(7):861-71. doi: 10.1111/j.1440-1827.1988.tb02358.x.
Five cases of gastric carcinoma of undifferentiated (gastric) type containing Paneth-like cells were studied by immunoelectron microscopy for lysozyme using two methods. The Paneth-like cells in these carcinomas had lost their cellular polarity and possessed electron-dense granules. These granules varied in size (0.5-3 micron in diameter), electron density and shape. Post-embedding method revealed immunolocalization of lysozyme in these granules in all cases, similar to that in normal Paneth cells. Pre-embedding method showed immunoreactivity diffusely or focally located in the cytoplasm, but this approach was unable to reveal immunoreactivity in the granules, a feature also common to normal cells. Our study therefore confirmed that Paneth-like carcinoma cells and normal Paneth cells had the same features, and indicated that undifferentiated-type gastric carcinoma could possess specially differentiated cells, providing further support that such carcinoma could express multipotentiality. The difference in the results obtained by the two immunoelectron microscopy methods was discussed.
采用两种方法，通过免疫电子显微镜对5例含有潘氏样细胞的未分化（胃）型胃癌进行了溶菌酶研究。这些癌组织中的潘氏样细胞失去了细胞极性，具有电子致密颗粒。这些颗粒在大小（直径0.5 - 3微米）、电子密度和形状上各不相同。包埋后法显示在所有病例中溶菌酶在这些颗粒中的免疫定位，类似于正常潘氏细胞中的情况。包埋前法显示免疫反应性弥漫或局灶性位于细胞质中，但这种方法无法显示颗粒中的免疫反应性，这也是正常细胞的常见特征。因此，我们的研究证实了潘氏样癌细胞和正常潘氏细胞具有相同的特征，并表明未分化型胃癌可能拥有特殊分化的细胞，进一步支持了这种癌可表现出多能性的观点。讨论了两种免疫电子显微镜方法所得结果的差异。
