Ohtani H
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
Tohoku J Exp Med. 1990 Apr;160(4):333-42. doi: 10.1620/tjem.160.333.
Ultrastructural characterization has been performed on eosinophilic granular cells occurring in gastric carcinomas. Of these, the present paper dealt with enterochromaffin (EC)-type cells. EC-type cells were identified by the presence of eosinophilic granules, immunoreactivity for serotonin (5 HT) and argentaffinity. Conventional electron microscopy revealed that they bore membrane bound, electron-dense granules of varying size (100-800 nm in shorter diameter), which were larger than those of normal EC cells. Their shapes are round, rod-shaped and concave. The granules are immunolabeled for 5HT by both pre- and post-embedding methods. The preembedding method also disclosed diffuse cytosolic immunoreactivity. Both differentiated-type and undifferentiated-type gastric carcinomas showed essentially the same results. General trends of occurrence of Paneth-like and EC-type cells in gastric carcinomas were also discussed. The present study confirmed that EC-type cells in gastric carcinoma retained basic characteristics of EC cells despite of morphological deviation from the normal cells. It is speculated that they are derived from neoplastic precursor cells with a special differentiation.
已对胃癌中出现的嗜酸性粒细胞进行了超微结构表征。其中，本文研究的是肠嗜铬（EC）细胞类型。通过嗜酸性颗粒的存在、对血清素（5-羟色胺）的免疫反应性和嗜银性来鉴定EC细胞类型。传统电子显微镜显示，它们含有膜结合的、大小不一（短径100-800纳米）的电子致密颗粒，这些颗粒比正常EC细胞的颗粒大。它们的形状为圆形、杆状和凹形。通过包埋前和包埋后方法，颗粒均被5-羟色胺免疫标记。包埋前方法还显示出弥漫性胞质免疫反应性。分化型和未分化型胃癌的结果基本相同。本文还讨论了胃癌中潘氏细胞样和EC细胞类型出现的总体趋势。本研究证实，胃癌中的EC细胞尽管在形态上与正常细胞有所偏差，但仍保留了EC细胞的基本特征。据推测，它们源自具有特殊分化的肿瘤前体细胞。
