Govindarajan Raghavan, Tejas Vyas, Pushpangadan Palpu
Research & Development Zydus Wellness Ltd, House No. 6 & 7, Sigma Commerce Zone, Nr. Iskcon Temple, S.G. Highway, Ahmedabad, Gujarat, India 380015.
Amity Institute for Herbal and Biotech Products Development, 3-Ravi Nagar, Peroorkada PO, Thiruvananthapuram, Kerala, India 695 005.
J AOAC Int. 2019 Jul 1;102(4):986-992. doi: 10.5740/jaoacint.18-0378. Epub 2018 Dec 17.
Herbal formulations have reached tremendous acceptability as therapeutic agents for several diseases mainly due to the indiscriminate use of modern medicine such as antibiotics, steroids and other synthetic drugs. The increasing popularity in plant-based drugs is leading to a fast growing market for plant-based drugs pharmaceuticals, nutraceuticals, functional foods, and even cosmeceuticals. The development of authentic analytical methods for complex herbal drugs, especially poly herbal formulations which can reliably profile the phytochemical composition, including quantitative analyses of marker/bioactive compounds and other major constituents, is a major challenge to scientists. Standardization is an important step for the establishment of a consistent biological activity, a consistent chemical profile, or simply a quality assurance program for production and manufacturing of herbal drugs. HPLC as a tool has been widely used in the standardization of complex herbal drugs because of its ability to estimate the presence of active (chemical or biological) markers both qualitatively and quantitatively. An overview of various HPLC techniques that can be used for standardization of herbal drugs has been presented.
草药配方作为多种疾病的治疗药物已获得极大的认可度，这主要归因于对抗生素、类固醇和其他合成药物等现代医学的滥用。植物性药物日益普及，使得基于植物的药物在制药、营养保健品、功能性食品乃至药妆品领域的市场迅速增长。开发针对复杂草药尤其是多草药配方的可靠分析方法，以准确描述其植物化学成分，包括对标志性/生物活性化合物及其他主要成分进行定量分析，是科学家面临的一项重大挑战。标准化是确立一致的生物活性、一致的化学特征，或者仅仅是为草药生产和制造建立质量保证计划的重要一步。由于能够定性和定量评估活性（化学或生物）标志物的存在，高效液相色谱法（HPLC）作为一种工具已被广泛应用于复杂草药的标准化。本文综述了可用于草药标准化的各种HPLC技术。
