Cui Guanglin, Ren Jingli, Xu Gang, Li Zhenfeng, Zheng Wei, Yuan Aping
1Research Group of Gastrointestinal Diseases, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan China.
2Faculty of Health Science, Nord University, Campus Levanger, Levanger, Norway.
Cancer Cell Int. 2018 Dec 11;18:203. doi: 10.1186/s12935-018-0700-2. eCollection 2018.
Emerging evidence has suggested that interleukin (IL)-33 and its primary functional receptor ST2 are involved in the pathogenesis of tumorigenesis.
Using immunohistochemistry (IHC) and double immunofluorescence staining, we characterized the cellular and clinicopathological features of the IL-33/ST2 axis in different compartments in human esophageal squamous cell carcinoma (ESCC) surgical specimens.
IHC data revealed an increased expression of IL-33-immunoreactivity (IR) and ST2-IR located in both ESCC cells and tumor stromal cells; which were associated with advanced clinicopathological features such as TNM stages and node involvement. However, the Kaplan-Meier analysis showed that densities of neither IL-33 positive nor ST2 positive cells in both the ESCC mass and stroma were associated with the overall survival rate in patients with ESCC. Double immunofluorescence staining for cellular feature analysis demonstrated that these IL-33 positive and ST2 positive cells in ESCCs were with a high proliferation rate, and IL-33-IR was frequently co-expressed with ST2-IR in both ESCC and stromal cells.
Significant altered cellular features of the IL-33/ST2 axis in ESCCs were associated with advanced clinicopathological variables. The data suggest that the IL-33/ST2 axis might be involved in the progression of human ESCCs.
新出现的证据表明，白细胞介素（IL）-33及其主要功能受体ST2参与肿瘤发生的发病机制。
我们采用免疫组织化学（IHC）和双重免疫荧光染色，对人食管鳞状细胞癌（ESCC）手术标本不同区域中IL-33/ST2轴的细胞和临床病理特征进行了表征。
免疫组织化学数据显示，ESCC细胞和肿瘤基质细胞中IL-33免疫反应性（IR）和ST2-IR的表达均增加；这与TNM分期和淋巴结受累等晚期临床病理特征相关。然而，Kaplan-Meier分析表明，ESCC肿块和基质中IL-33阳性细胞和ST2阳性细胞的密度均与ESCC患者的总生存率无关。用于细胞特征分析的双重免疫荧光染色表明，ESCC中的这些IL-33阳性细胞和ST2阳性细胞增殖率高，并且IL-33-IR在ESCC和基质细胞中经常与ST2-IR共表达。
ESCC中IL-33/ST2轴的细胞特征显著改变与晚期临床病理变量相关。数据表明，IL-33/ST2轴可能参与人类ESCC的进展。
