Center for Neuro-Medicine, Brain Science Institute , Korea Institute of Science and Technology , Seoul 02792 , Korea.
Bioconjug Chem. 2019 Jan 16;30(1):210-217. doi: 10.1021/acs.bioconjchem.8b00845. Epub 2019 Jan 4.
Herein, we developed a near-infrared (NIR) fluorescent probe for mitochondrial staining based on the NIR fluorochrome, silicon-rhodamine. The hydrophobicity of the fluorescent core was systematically modified by conjugation with 10 different commercial amines. The resulting fluorescent compounds exhibited similar photophysical properties but diverse hydrophobicity. We identified the optimal level of hydrophobicity associated with high mitochondrial targeting efficiency. In particular, the SiR-Mito 8 probe provided excellent mitochondrial staining and successfully differentiated the live Hep3B cancer cells from normal L02 cells in vitro.
在此，我们基于近红外荧光染料硅罗丹明开发了一种用于线粒体染色的近红外荧光探针。通过与 10 种不同的商业胺连接，系统地修饰了荧光核的疏水性。所得荧光化合物表现出相似的光物理性质，但疏水性不同。我们确定了与高线粒体靶向效率相关的最佳疏水性水平。特别是 SiR-Mito 8 探针提供了出色的线粒体染色效果，并成功地区分了体外的活 Hep3B 癌细胞与正常 L02 细胞。
