Kalinichenko Sergei G, Matveeva Natalya Yu, Kostiv Roman Ye, Edranov Sergey S
Department of Histology, Cytology and Embryology, Pacific State Medical University, Vladivostok, Russia.
Biomed Mater Eng. 2019;30(1):85-95. doi: 10.3233/BME-181035.
Biodegradable implant coatings promote proliferation and expression of BMP-2, VEGF, and TGF-β2 genes and enhance BMP-2, VEGF, and TGF-β2 regulatory effects at different stages of reparative osteogenesis.
To study the topography and ratio of PCNA-, VEGF-, BMP-2-, and TGF-β2-immunoreactive cells in rat femoral bone after closed fracture and implantation of titanium implants with biodegradable calcium phosphate and hydroxyapatite coatings.
Standard titanium implant screws and similar implants with bioactive coatings were used. A total of 18 rats were randomly divided into three groups, two experimental and a control one. The rats in the first experimental group were implanted with implants without specific coating, while those in the second group, with implants with specific coatings. The control rats were subjected to the same fracture as the experimental ones without subsequent implantation. On days 7, 14, and 30 of experiment, the rats were sampled for histological examination. Histological sections were prepared and processed for PCNA, BMP-2, VEGF, and TGF-β2 immunoreactivity.
In the regeneration zone, PCNA-immunoreactive cells substantially outnumbered other immunoreactive cell types. During the first two weeks after fracture, in the immediate vicinity of implant surface, the rate of VEGF production increased in osteoblast subpopulations and level of TGF-32 immunoreactivity decreased in chondroblasts. The level of TGF-32 was maximum on day 30 of experiment. BMP-2-immunoreactive osteocytes were found in the zone of external general plates. They accumulated at implants with calcium phosphate coating. Their number gradually increased by day 30 of experiment.
The present data suggest that biodegradable implant coatings promote proliferation and expression of BMP-2, VEGF, and TGF-β2 genes and enhance BMP-2, VEGF, and TGF-β2 regulatory effects at different stages of reparative osteogenesis.
可生物降解的植入物涂层可促进BMP-2、VEGF和TGF-β2基因的增殖和表达，并增强其在修复性骨生成不同阶段的调节作用。
研究闭合性骨折及植入具有可生物降解磷酸钙和羟基磷灰石涂层的钛植入物后，大鼠股骨中PCNA、VEGF、BMP-2和TGF-β2免疫反应性细胞的形态和比例。
使用标准钛植入螺钉和具有生物活性涂层的类似植入物。将18只大鼠随机分为三组，两组为实验组，一组为对照组。第一实验组的大鼠植入无特定涂层的植入物，而第二组大鼠植入有特定涂层的植入物。对照大鼠与实验大鼠遭受相同的骨折，但不进行后续植入。在实验的第7、14和30天，对大鼠进行取样以进行组织学检查。制备组织学切片并进行PCNA、BMP-2、VEGF和TGF-β2免疫反应性处理。
在再生区，PCNA免疫反应性细胞的数量明显多于其他免疫反应性细胞类型。骨折后的前两周内，在植入物表面附近，成骨细胞亚群中VEGF的产生率增加而软骨细胞中TGF-32免疫反应性水平降低。在实验第30天时TGF-32水平最高。在外部总板区域发现了BMP-2免疫反应性骨细胞。它们在有磷酸钙涂层的植入物处聚集。到实验第30天时其数量逐渐增加。
目前的数据表明，可生物降解的植入物涂层可促进BMP-2、VEGF和TGF-β2基因的增殖和表达，并增强其在修复性骨生成不同阶段的调节作用。
