Bi Xiangdong, Maturavongsadit Panita, Tan Yu, Watts Morgan, Bi Evelyn, Kegley Zachary, Morton Steve, Lu Lin, Wang Qian, Liang Aiye
Department of Physical Sciences, Charleston Southern University, Charleston, South Carolina, USA.
Department of Chemistry and Biochemistry, University of South Carolina, Columbia, South Carolina, USA.
Biomed Mater Eng. 2019;30(1):111-123. doi: 10.3233/BME-181037.
Biocompatible hydrogel systems with tunable mechanical properties have been reported to influence the behavior and differentiation of mesenchymal stem cells (MSCs).
To develop a functionalized hydrogel system with well-defined chemical structures and tunable mechanical property for regulation of stem cell differentiation.
An in situ-forming hydrogel system is developed by crosslinking vinyl sulfone functionalized polyamidoamine (PAMAM) dendrimer and multi-armed thiolated polyethylene glycol (PEG) through a thiol-ene Michael addition in aqueous conditions. The viability and differentiation of MSCs in hydrogels of different stiffness are conducted for 21 days under corresponding induction media.
MSCs are viable in synthesized hydrogels after 48 hours of culture. By varying the concentrations of PAMAM dendrimer and PEG, hydrogels of different gelation time and stiffness are achieved. The MSC differentiation indicates that more osteogenic differentiation is observed in hard gel (5,663 Pa) and more adipogenic differentiation is observed in soft gel (77 Pa) in addition to the differentiation caused by each individual induction media during the process of culture.
A biocompatible in situ-forming hydrogel system is successfully synthesized. This hydrogel system has shown influences on differentiation of MSCs and may potentially be important in developing therapeutic strategies in medical applications.
据报道，具有可调机械性能的生物相容性水凝胶系统会影响间充质干细胞（MSC）的行为和分化。
开发一种具有明确化学结构和可调机械性能的功能化水凝胶系统，用于调节干细胞分化。
通过在水性条件下，使乙烯基砜功能化的聚酰胺-胺（PAMAM）树枝状大分子与多臂硫醇化聚乙二醇（PEG）通过硫醇-烯迈克尔加成反应交联，开发一种原位形成的水凝胶系统。在相应的诱导培养基中，对不同硬度水凝胶中的MSC进行21天的活力和分化研究。
培养48小时后，MSC在合成水凝胶中具有活力。通过改变PAMAM树枝状大分子和PEG的浓度，可获得具有不同凝胶化时间和硬度的水凝胶。MSC分化表明，在培养过程中，除了每种单独的诱导培养基引起的分化外，在硬凝胶（5663 Pa）中观察到更多的成骨分化，在软凝胶（77 Pa）中观察到更多的成脂分化。
成功合成了一种生物相容性原位形成的水凝胶系统。该水凝胶系统已显示出对MSC分化的影响，可能在医学应用中开发治疗策略方面具有重要意义。
