Yusuf Mohamed S, El Nabtiti Adel A, Hassan Marwa A, Mandour Mostafa A
Department of Nutrition and Clinical Nutrition, Suez Canal University, Egypt.
Department of Animal Wealth Development (Animal Production Division), Suez Canal University, Egypt.
Int J Vet Sci Med. 2018 Nov 22;6(2):213-218. doi: 10.1016/j.ijvsm.2018.11.004. eCollection 2018 Dec.
Egypt's population is growing with the biggest hurdle facing the Government is to secure animal protein. Broilers provide quality protein of reasonable price. This study was conducted to investigate the outcomes of dietary organic betaine (betafin S4) on productive, epigenetic make up of lipoprotein lipase gene (LPL) promoter, some blood biochemical, and economic parameters in male broilers at finishing period. Eighty one commercial Arbor Acre Plus males, 21 days old, were randomly allocated to three groups, with three replicates each in battery cages under thermo-neutral environment till 42 days. The examined groups received yellow corn-soy basal diet, supplemented with 0 (G1), 1.5 (G2) and 3.0 g (G3) betaine/kg diet, respectively. The mRNA expression levels of LPL gene were analyzed by real-time quantitative PCR. Methylation pattern on LPL gene promoter was determined by bisulfite sequencing. Doses of betaine statistically ( ≤ .05) improved tested performance parameters; while carcass yield % and abdominal fat deposition did not achieve significant changes. The expression of LPL mRNA showed an inverse relationship with betaine dose, which illustrated as a trend toward increase in G2 and decrease in G3. Regarding serum biochemistry, both treated groups when compared to control group revealed a significant improvement ( ≤ .01) in albumin level, simultaneously, a significant increase ( ≤ .05) was recorded in uric acid and triglyceride levels, additionally, strong positive ( ≤ .01) correlation between betaine dose and previously mentioned parameters was reported. Betaine is recommended in finishing male broilers as production costs were reduced by 3.97%-4.37% per kg, respectively. In conclusion, incorporation of 0.15-0.30% organic betaine to male broilers diets during finishing period improves the growth performances.
埃及人口不断增长，政府面临的最大障碍是确保动物蛋白供应。肉鸡能提供价格合理的优质蛋白。本研究旨在探讨日粮添加有机甜菜碱（倍他芬S4）对雄性肉鸡育肥期生产性能、脂蛋白脂肪酶基因（LPL）启动子的表观遗传组成、一些血液生化指标及经济参数的影响。81只21日龄的商业艾拔益加雄性肉鸡被随机分为三组，每组三个重复，置于热中性环境下的层叠式笼中饲养至42日龄。试验组分别接受添加0（G1）、1.5（G2）和3.0克（G3）甜菜碱/千克日粮的玉米-豆粕基础日粮。通过实时定量PCR分析LPL基因的mRNA表达水平。采用亚硫酸氢盐测序法测定LPL基因启动子的甲基化模式。甜菜碱剂量在统计学上（≤.05）改善了试验性能参数；而胴体产率%和腹部脂肪沉积未出现显著变化。LPL mRNA的表达与甜菜碱剂量呈负相关，表现为G2组有升高趋势，G3组有下降趋势。关于血清生化指标，与对照组相比，两个处理组的白蛋白水平均有显著改善（≤.01），同时，尿酸和甘油三酯水平显著升高（≤.05），此外，甜菜碱剂量与上述参数之间存在强正相关（≤.01）。建议在雄性肉鸡育肥期使用甜菜碱，因为每千克生产成本分别降低了3.97%-4.37%。总之，在雄性肉鸡育肥期日粮中添加0.15%-0.30%的有机甜菜碱可提高生长性能。
