Department of Thoracic Surgery, West China Hospital of Sichuan University, Chengdu, China.
North Sichuan Medical College, Nanchong, China.
J Cell Mol Med. 2019 Feb;23(2):1375-1385. doi: 10.1111/jcmm.14040. Epub 2018 Dec 18.
FAM3B has been suggested to play important roles in the progression of many cancers, such as gastric, oral, colon and prostate cancer. However, little is known about the role of FAM3B in human esophageal squamous cell carcinoma (ESCC). In the present study, we found that FAM3B expression was higher in ESCC tissues than in adjacent normal tissues. Using quantitative real-time polymerase chain reaction, we found similar results in cell lines. FAM3B expression was significantly related to T/TNM stage. Importantly, Kaplan-Meier analysis revealed that a high expression level of FAM3B predicted a poor outcome for ESCC patients. Overexpression of FAM3B inhibits ESCC cell death, increases oesophageal tumour growth in xenografted nude mice, and promotes ESCC cell migration and invasion. Further studies confirmed that FAM3B regulates the AKT-MDM2-p53 pathway and two core epithelial-to-mesenchymal transition process markers, Snail and E-cadherin. Our results provide new insights into the role of FAM3B in the progression of ESCC and suggest that FAM3B may be a promising molecular target and diagnostic marker for ESCC.
FAM3B 被认为在许多癌症的进展中发挥重要作用，如胃癌、口腔癌、结肠癌和前列腺癌。然而，关于 FAM3B 在人类食管鳞状细胞癌（ESCC）中的作用知之甚少。在本研究中，我们发现 FAM3B 在 ESCC 组织中的表达高于相邻正常组织。通过实时定量聚合酶链反应，我们在细胞系中也得到了类似的结果。FAM3B 的表达与 T/TNM 分期显著相关。重要的是，Kaplan-Meier 分析表明 FAM3B 高表达预示 ESCC 患者预后不良。过表达 FAM3B 抑制 ESCC 细胞死亡，增加异种移植裸鼠食管肿瘤生长，并促进 ESCC 细胞迁移和侵袭。进一步的研究证实，FAM3B 调节 AKT-MDM2-p53 通路和两个核心上皮间质转化过程标志物，Snail 和 E-cadherin。我们的研究结果为 FAM3B 在 ESCC 进展中的作用提供了新的见解，并表明 FAM3B 可能是 ESCC 有前途的分子靶点和诊断标志物。
