分娩及产后期间MicroRNA-210的表达作为急性胎儿缺氧的潜在生物标志物
MicroRNA-210 expression during childbirth and postpartum as a potential biomarker of acute fetal hypoxia.
作者信息
Vonkova Barbara, Blahakova Ivona, Hruban Lukas, Janku Petr, Pospisilova Sarka
机构信息
Center of Molecular Medicine, CEITEC - Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Center of Molecular Biology and Gene Therapy, Department of Internal Medicine - Hematology and Oncology, University Hospital Brno, Czech Republic.
出版信息
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2019 Sep;163(3):259-264. doi: 10.5507/bp.2018.075. Epub 2018 Dec 18.
OBJECTIVE
To explore whether miR-210 expression can be used as a diagnostic and prognostic marker in acute fetal hypoxia.
METHODS
Whole blood samples of 29 women and their fetuses without hypoxia and 24 women and their fetuses with hypoxia were analysed in this study. Reverse transcription and quantitative real-time PCR were used to measure the expression of miR-210. Expression level differences between the control and hypoxic group in labour time and postpartum change fold were analyzed by standard statistical tests.
RESULTS
We confirmed that miR-210 is significantly more upregulated in fetal blood with acute hypoxia when compared to maternal blood (P Conclusions: Our study confirmed miR-210 upregulation in the blood of pregnant women with acute fetal hypoxia at the time of labour compared to pregnant women without acute fetal hypoxia. Additional investigation should be done to determine miR-210 clearance and the possibility of using miR-210 as a diagnostic and prognostic marker.
目的
探讨miR-210表达是否可作为急性胎儿缺氧的诊断和预后标志物。
方法
本研究分析了29名无缺氧的孕妇及其胎儿和24名有缺氧的孕妇及其胎儿的全血样本。采用逆转录和定量实时PCR检测miR-210的表达。通过标准统计检验分析对照组和缺氧组在分娩时间和产后变化倍数方面的表达水平差异。
结果
我们证实，与母体血液相比，急性缺氧时胎儿血液中miR-210的上调更为显著（P结论：我们的研究证实，与无急性胎儿缺氧的孕妇相比，有急性胎儿缺氧的孕妇在分娩时血液中miR-210上调。应进一步研究以确定miR-210的清除情况以及将其用作诊断和预后标志物的可能性。
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