Beijing National Research Center for Molecular Sciences, Key Laboratory of Molecular Nanostructure and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, P. R. China.
University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Adv Healthc Mater. 2018 Dec;7(24):e1801231. doi: 10.1002/adhm.201801231. Epub 2018 Nov 22.
Detection of circulating tumor cells (CTCs) in peripheral blood is of paramount significance for cancer diagnosis, progress evaluation, and individualized therapy. However, the rareness and heterogeneity of CTCs introduces significant challenges in the capture of cancer cells as well as downstream genetic analysis. In this work, a microwell-assisted multiaptamer immunomagnetic platform (MMAIP) is proposed for highly efficient capture of CTCs with minimum influence of heterogeneity. Assisted by a microwell chip, the purity of CTCs is greatly improved, thus meeting the requirement of downstream gene analysis. This is, as far as is known, the first aptamer based platform enabling mutation analysis of the captured CTCs from cancer patients, which will contribute to the practical application of aptamers in clinics.
检测外周血中的循环肿瘤细胞（CTC）对于癌症诊断、进展评估和个体化治疗至关重要。然而，CTC 的稀有性和异质性给癌症细胞的捕获以及下游的基因分析带来了重大挑战。在这项工作中，提出了一种基于微孔辅助多适体免疫磁珠平台（MMAIP）的方法，用于高效捕获 CTC，同时最大限度地减少异质性的影响。在微孔芯片的辅助下，CTC 的纯度得到了极大的提高，从而满足了下游基因分析的要求。据了解，这是第一个基于适体的平台，能够对癌症患者捕获的 CTC 进行突变分析，这将有助于适体在临床上的实际应用。
