The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, The Key Laboratory of Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, Collaborative Innovation Center of Chemistry for Energy Materials, Department of Chemical Biology, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, 361005, China.
Center for Soft Condensed Matter Physics and Interdisciplinary Research, School of Physical Science and Technology, Soochow University, Suzhou, 215021, China.
Adv Sci (Weinh). 2021 Oct;8(20):e2102070. doi: 10.1002/advs.202102070. Epub 2021 Sep 2.
Controllable assembly and disassembly of recognition interface are vital for bioanalysis. Herein, a strategy of dynamic manipulation of trapping force by engineering a dynamic and reversible immunoaffinity microinterface (DynarFace) in a herringbone chip (DynarFace-Chip) for liquid biopsy is proposed. The DynarFace is assembled by magnetically attracting immunomagnetic beads (IMBs) on chip substrate, with merits of convenient operation and reversible assembly. The DynarFace allows accumulating attachment of IMBs on circulating rare cell (CRC) surfaces during hydrodynamically enhanced interface collision, where accumulatively enhanced magnetic trapping force improves capture efficiency toward CRCs with medium expression of biomarkers from blood samples by 134.81% compared with traditional non-dynamic interfaces. Moreover, magnet withdrawing-induced disappearance of trapping force affords DynarFace disassembly and CRC release with high efficiency (>98%) and high viability (≈98%), compatible with downstream in vitro culture and gene analysis of CRCs. This DynarFace strategy opens a new avenue to accumulated capture and reversible release of CRCs, holding great potential for liquid biopsy-based precision medicine.
可控的识别界面组装和拆卸对于生物分析至关重要。在这里，我们提出了一种通过在人字形芯片（DynarFace-Chip）中构建动态且可逆的免疫亲和微界面（DynarFace）来动态操纵捕获力的策略，用于液体活检。DynarFace 通过在芯片基底上磁吸引免疫磁珠（IMB）来组装，具有操作方便和可重复组装的优点。在增强的界面碰撞过程中，DynarFace 允许在循环稀有细胞（CRC）表面上累积地附着 IMB，在增强的磁场捕获力的作用下，与传统的非动态界面相比，从中提取的具有中等生物标志物表达的血液样本中的 CRC 的捕获效率提高了 134.81%。此外，磁体撤出诱导的捕获力消失提供了 DynarFace 的高效（>98%）和高活力（≈98%）的拆卸和 CRC 释放，与 CRC 的下游体外培养和基因分析兼容。这种 DynarFace 策略为 CRC 的累积捕获和可逆释放开辟了新途径，为基于液体活检的精准医疗提供了巨大潜力。
