Department of Resources Science of Traditional Chinese Medicines, School of Traditional Chinese Pharmacy, China Pharmaceutical University, #24 Tongjiaxiang, Gulou District, Nanjing 210009, China.
Jiangsu Key Laboratory of Regional Resource Exploitation and Medicinal Research, Huaiyin Institute of Technology, Huai'an 223003, Jiangsu, China.
Molecules. 2018 Dec 18;23(12):3359. doi: 10.3390/molecules23123359.
Pall. var. (Fisch.) Koidz. is a traditional medicinal plant resource. To make full use of the plant resources, constituents of leaves were determined by high performance liquid chromatography (HPLC)-quadrupole time-of-flight tandem mass spectrometry and 22 -glycosylflavones were identified or tentatively identified. Optimal extraction of leaves was established via single factor investigations combined with response surface methodology. Then, HPLC coupled with a diode array detector was used to quantitatively analyze the six main components of 14 batches of leaves grown in different areas. The results showed the -glycosylflavones were the main components of leaves, and the total contents of detected components were relatively stable for the majority of samples. These results provide a foundation for the development and utilization of leaves.
帕氏变种（Fisch.）Koidz. 是一种传统药用植物资源。为了充分利用植物资源，采用高效液相色谱-四极杆飞行时间串联质谱法测定了叶片的成分，并鉴定或初步鉴定了 22 种糖基黄酮。通过单因素研究结合响应面法建立了叶片的最佳提取条件。然后，采用高效液相色谱法结合二极管阵列检测器对 14 批不同产地的叶片的 6 种主要成分进行定量分析。结果表明，糖基黄酮是叶片的主要成分，大多数样品中检测成分的总含量相对稳定。这些结果为 叶片的开发和利用提供了基础。
