Thao Nguyen Phuong, Luyen Bui Thi Thuy, Widowati Wahyu, Fauziah Nurul, Maesaroh Maesaro, Herlina Tati, Manzoor Zahid, Ali Irshad, Koh Young Sang, Kim Young Ho
College of Pharmacy, Chungnam National University, Daejeon, Republic of Korea.
Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Planta Med. 2016 Nov;82(17):1475-1481. doi: 10.1055/s-0042-108737. Epub 2016 Jun 10.
Four new compounds, acacetin 8-C-[-D-apiofuranosyl-(1 → 2)--D-glucopyranoside] (), 7-methoxyacacetin 8-C-[-D-apiofuranosyl-(1 → 3)--D-glucopyranoside] (), 7-methoxyacacetin 8-C-[-D-glucopyranosyl-(1 → 2)--D-glucopyranoside] (), and 4‴-O-acetylacacetin 8-C-[-L-rhamnopyranosyl-(1 → 2)--D-glucopyranoside] (), along with ten known compounds (-), were isolated from leaves. The effects of these compounds on lipopolysaccharide-induced expression of the proinflammatory cytokines IL-12 p40, IL-6, and TNF- in bone marrow-derived dendritic cells were evaluated. Compounds , and - inhibited the production of both IL-12 p40 and IL-6, with IC values ranging from 0.35 ± 0.01 to 1.40 ± 0.04 µM and 1.22 ± 0.02 to 3.79 ± 0.10 µM, respectively. Compounds and only showed strong inhibition effects on the production of IL-12 p40, with IC values of 2.76 ± 0.08 and 0.39 ± 0.05 µM, respectively. However, all compounds showed weak activity or no activity on TNF- production at the tested concentrations.
从 叶中分离出四种新化合物，刺槐素 8 - C - [-D - 阿糖呋喃糖基 - (1→2)-β - D - 吡喃葡萄糖苷] ()、7 - 甲氧基刺槐素 8 - C - [-D - 阿糖呋喃糖基 - (1→3)-β - D - 吡喃葡萄糖苷] ()、7 - 甲氧基刺槐素 8 - C - [-D - 吡喃葡萄糖基 - (1→2)-β - D - 吡喃葡萄糖苷] () 和 4‴ - O - 乙酰基刺槐素 8 - C - [-L - 鼠李吡喃糖基 - (1→2)-β - D - 吡喃葡萄糖苷] ()，以及十种已知化合物 (-)。评估了这些化合物对脂多糖诱导的骨髓来源树突状细胞中促炎细胞因子 IL - 12 p40、IL - 6 和 TNF - 的表达的影响。化合物 、 和 - 抑制了 IL - 12 p40 和 IL - 6 的产生，IC 值分别为 0.35±0.01 至 1.40±0.04 μM 和 1.22±0.02 至 3.79±0.10 μM。化合物 和 仅对 IL - 12 p40 的产生显示出强烈的抑制作用，IC 值分别为 2.76±0.08 和 0.39±0.05 μM。然而，在测试浓度下，所有化合物对 TNF - 的产生均显示出弱活性或无活性。
