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来自发光杆菌的高度重复的tRNA(脯氨酸)-tRNA(组氨酸)基因簇。

Highly repetitive tRNA(Pro)-tRNA(His) gene cluster from Photobacterium phosphoreum.

作者信息

Giroux S, Beaudet J, Cedergren R

机构信息

Département de Biochimie, Université de Montréal, Québec, Canada.

出版信息

J Bacteriol. 1988 Dec;170(12):5601-6. doi: 10.1128/jb.170.12.5601-5606.1988.

DOI:10.1128/jb.170.12.5601-5606.1988
PMID:3056906
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC211657/
Abstract

A DNA fragment comprising the four tRNA gene sequences of the Escherichia coli argT locus hybridized with two Sau3A-generated DNA fragments from the vibrio Photobacterium phosphoreum (ATCC 11040). Detailed sequence analysis of the longer fragment shows the following gene organization: 5'-promoter-tRNA(Pro)-tRNAPro-tRNA(Pro)-tRNA(His)-tRNA(Pro)-tRNA(Pro)- tRNA(His)-tRNA(Pro)-five pseudogenes derived from the upstream tRNAPro interspersed by putative Rho-independent terminators. This sequence demonstrates the presence of highly repetitive, tandem tRNA genes in a bacterial genome. Furthermore, a stretch of 304 nucleotides from this cluster was found virtually unchanged in the other (shorter) fragment which was previously sequenced. The two clusters together contain eight tRNA(Pro) pseudogenes and eight fully intact tRNA(Pro) genes, an unusually high number for a single eubacterial isoacceptor tRNA. These results show that the organization of some tRNA operons is highly variable in eubacteria.

摘要

一个包含大肠杆菌argT基因座四个tRNA基因序列的DNA片段,与来自磷光弧菌(ATCC 11040)的两个Sau3A酶切产生的DNA片段杂交。对较长片段的详细序列分析显示了以下基因组织:5'-启动子-tRNA(Pro)-tRNAPro-tRNA(Pro)-tRNA(His)-tRNA(Pro)-tRNA(Pro)-tRNA(His)-tRNA(Pro)-五个源自上游tRNAPro的假基因,其间穿插着假定的不依赖Rho的终止子。该序列证明了细菌基因组中存在高度重复的串联tRNA基因。此外,在先前测序的另一个(较短)片段中发现,来自该簇的一段304个核苷酸的序列几乎没有变化。这两个簇总共包含八个tRNA(Pro)假基因和八个完全完整的tRNA(Pro)基因,对于单个真细菌同功受体tRNA来说,这个数量异常高。这些结果表明,真细菌中一些tRNA操纵子的组织具有高度变异性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/116c96463e54/jbacter00190-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/85dabab0eaf0/jbacter00190-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/4d687f6f9134/jbacter00190-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/116c96463e54/jbacter00190-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/85dabab0eaf0/jbacter00190-0210-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/4d687f6f9134/jbacter00190-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/babc/211657/116c96463e54/jbacter00190-0213-a.jpg

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本文引用的文献

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The hisR locus of Salmonella: nucleotide sequence and expression.沙门氏菌的hisR基因座:核苷酸序列与表达
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Repeated sequences and open reading frames in the 3' flanking region of the gene for the RNA subunit of Escherichia coli ribonuclease P.大肠杆菌核糖核酸酶P的RNA亚基基因3'侧翼区域中的重复序列和开放阅读框。
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Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis.利用寡脱氧核苷酸定向诱变构建改良的M13载体。
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Conformational change in the DNA associated with an unusual promoter mutation in a tRNA operon of Salmonella.与沙门氏菌tRNA操纵子中异常启动子突变相关的DNA构象变化。
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