Welsh J, McClelland M
California Institute of Biological Research, La Jolla 92037.
Nucleic Acids Res. 1991 Feb 25;19(4):861-6. doi: 10.1093/nar/19.4.861.
The polymerase chain reaction using only a single 'consensus' tRNA gene primer, or a pair of primers facing outward from tRNA genes, amplifies a set of DNA fragments in bacterial, plant and animal genomic DNAs. Presumably, these PCR fingerprints are mainly derived from the regions between closely linked tRNA genes. The pattern of the PCR products is determined by which genomes and which primer(s) are used. Genomic fingerprints are largely conserved within a species and, in bacteria, most products in the fingerprint are conserved between closely related species. Thus, PCR with tRNA gene consensus primers helps to identify species and genera.
使用仅单个“共有”tRNA基因引物或一对从tRNA基因向外的引物进行聚合酶链反应，可在细菌、植物和动物基因组DNA中扩增出一组DNA片段。据推测，这些PCR指纹图谱主要源自紧密相连的tRNA基因之间的区域。PCR产物的模式取决于所使用的基因组和引物。基因组指纹图谱在一个物种内基本保守，并且在细菌中，指纹图谱中的大多数产物在密切相关的物种之间是保守的。因此，使用tRNA基因共有引物进行PCR有助于鉴定物种和属。
