Jones C J, Benbow E W, Knox W F, Stoddart R W
Pathology Department, University of Manchester.
J Clin Pathol. 1988 Sep;41(9):1017-8. doi: 10.1136/jcp.41.9.1017.
Using biotinylated Arachis hypogaea agglutinin (AHA) or peanut lectin (PNA) and an avidin-peroxidase procedure, the various stages of spermatid development were visualised with great clarity; a light haematoxylin counterstain permitted the easy recognition of spermatogenic cells in the same section. This method is particularly useful in the interpretation of poorly fixed material and may be helpful in studies of cyclical maturation of spermatozoa, irrespective of whether the material is obtained at biopsy or necropsy.
使用生物素化的花生凝集素（AHA）或花生凝集素（PNA）以及抗生物素蛋白-过氧化物酶程序，精子细胞发育的各个阶段都能非常清晰地显现出来；用苏木精进行轻度复染可以在同一切片中轻松识别生精细胞。这种方法在解释固定不佳的材料时特别有用，并且可能有助于精子周期性成熟的研究，无论材料是通过活检还是尸检获得的。
