Jones C J, Morrison C A, Stoddart R W
Department of Pathological Sciences, University of Manchester, UK.
Histochem J. 1992 Jun;24(6):327-36. doi: 10.1007/BF01046164.
Rat testes have been examined with a panel of lectins that bind specifically to oligosaccharide sequences having terminal or subterminal beta-galactosyl residues in O-linked glycans, or in the outer chains of complex N-linked glycans: Arachis hypogaea (peanut, AHA), Erythrina cristagalli (coral tree, ECA), Ricinus communis (castor bean, RCA120) and Abrus precatorius (jequirity bean, APA) agglutinins. Pretreatment of sections with neuraminidase, beta-galactosidase and removal of alkali-labile O-linked sequences by beta-elimination allowed the structure of these glycans to be further explored. In spermatogonia and spermatocytes there was little evidence of glycans terminating in beta-galactosyl residues, although these were present at non-reducing terminals as sialylgalactosides. The acrosome contained two subsets of O-linked glycans terminating in sialylgalactosides, while the nuclear cap showed at least two subsets of N-linked sialylgalactosyl as well as O-linked glycans. Spermatozoa exhibited minor changes in the pattern of glycosylation, although the overall pattern of beta-galactosyl expression was similar. Binding to Sertoli cells showed the presence of some unsubstituted beta-galactosyl terminals on O-linked glycans but few such N-linked residues, while terminal beta-galactosides were scanty in tubular basement membranes.
已用一组凝集素对大鼠睾丸进行了检测，这些凝集素可特异性结合在O-连接聚糖或复杂N-连接聚糖外链中具有末端或亚末端β-半乳糖基残基的寡糖序列：花生（Arachis hypogaea，AHA）、刺桐（Erythrina cristagalli，ECA）、蓖麻（Ricinus communis，RCA120）和相思豆（Abrus precatorius，APA）凝集素。用神经氨酸酶、β-半乳糖苷酶预处理切片，并通过β-消除去除碱不稳定的O-连接序列，从而进一步探究这些聚糖的结构。在精原细胞和精母细胞中，几乎没有证据表明存在以β-半乳糖基残基为末端的聚糖，尽管这些聚糖以唾液酸半乳糖苷的形式存在于非还原末端。顶体含有两个以唾液酸半乳糖苷为末端的O-连接聚糖亚群，而核帽显示至少有两个N-连接唾液酸半乳糖基以及O-连接聚糖亚群。精子在糖基化模式上表现出微小变化，尽管β-半乳糖基表达的总体模式相似。与支持细胞的结合表明，O-连接聚糖上存在一些未取代的β-半乳糖基末端，但N-连接残基很少，而在肾小管基底膜中末端β-半乳糖苷很少。
