Toll样受体4（TLR4）中的单核苷酸多态性与HIV-1感染风险相关。

A single-nucleotide polymorphism in TLR4 is linked with the risk of HIV-1 infection.

作者信息

Vidyant S, Chatterjee A, Dhole T N

机构信息

a Department of Microbiology , Sanjay Gandhi Post Graduate Institute of Medical Sciences , Lucknow , India.

b Department of Biomedical sciences , Texas Tech University Health Sciences center , El Paso , TX , USA.

出版信息

Br J Biomed Sci. 2019 Apr;76(2):59-63. doi: 10.1080/09674845.2018.1559486. Epub 2019 Feb 11.

DOI:10.1080/09674845.2018.1559486

PMID:30569830

Abstract

INTRODUCTION

Toll-like receptors (TLRs) are pattern recognition receptors that play a role in innate immunity. Mounting evidence shows that single-nucleotide polymorphisms (SNPs) in TLRs link to various infectious diseases, including human immunodeficiency virus (HIV). We hypothesized links between two TLR4 SNPs (rs4986790 leading to Asp299Gly and rs4986791 leading to Thr399Ile) and HIV, to investigate the frequency of TLR4 polymorphism and its role in patients infected with HIV.

MATERIALS AND METHODS

We recruited 160 HIV-1 seropositive patients, who were further divided on disease severity based on CD4 count (stages I, II and III), and 270 age- and sex matched healthy HIV-1 seronegative individuals. Subjects were genotyped for TLR4 gene polymorphism by polymerase chain reaction restriction fragment length polymorphism.

RESULTS

The TLR4 Asp299Gly heterozygous genotype (OR=2.160; p=0.004) and the mutant allele G (OR=2.051; p=0.002) was higher in HIV-1 infection than healthy controls and also in stage I (OR=2.559; p=0.034) compared to different clinical stages of infection. There was no link between the Thr399Ile polymorphism and HIV infection.

CONCLUSION

The TLR4 (Asp299Gly) SNP is a risk factor in HIV-1 disease susceptibility.

摘要

引言

Toll样受体（TLR）是在先天免疫中起作用的模式识别受体。越来越多的证据表明，TLR中的单核苷酸多态性（SNP）与包括人类免疫缺陷病毒（HIV）在内的各种传染病相关。我们推测TLR4的两个SNP（导致Asp299Gly的rs4986790和导致Thr399Ile的rs4986791）与HIV之间存在关联，以研究TLR4多态性的频率及其在HIV感染患者中的作用。

材料与方法

我们招募了160名HIV-1血清阳性患者，根据CD4计数（I、II和III期）进一步按疾病严重程度进行分组，并招募了270名年龄和性别匹配的健康HIV-1血清阴性个体。通过聚合酶链反应-限制性片段长度多态性对受试者的TLR4基因多态性进行基因分型。

结果

与健康对照相比，HIV-1感染中TLR4 Asp299Gly杂合基因型（OR = 2.160；p = 0.004）和突变等位基因G（OR = 2.051；p = 0.002）更高，与不同临床感染阶段相比，I期（OR = 2.559；p = 0.034）中也是如此。Thr399Ile多态性与HIV感染之间没有关联。