Tang B Qing, Li Tongju, Bai Xuemei, Zhao Minyi, Wang Bing, Rein Glen, Yang Yongdong, Gao Peng, Zhang Xiaohuan, Zhao Yanpeng, Feng Qian, Cai Zhongzhen, Chen Yu
a Institute of Life Science and Technology , ENN group , Langfang , China.
b Quantum Biology Research Lab , Ridgway , CO , USA.
Electromagn Biol Med. 2019;38(1):55-65. doi: 10.1080/15368378.2018.1558064. Epub 2018 Dec 20.
DNA transduction across aqueous solutions has been reported previously. In this study, we examined a few key factors affecting DNA transduction rate in an extremely low frequency electromagnetic field. These include: the chemical composition of the aqueous solutions, the type of experimental vessel, the dilution step, and the origin of the DNA fragments. The results indicate that partially introducing essential ingredients for DNA amplification (i.e. dNTPs and PCR buffer) to the aqueous solution enhanced the transduction rate greatly, and transduction vessels made of hydrophilic quartz yielded more favorable results than vessels made of hydrophobic plastic. In addition, performing a serial dilution to the transduction solution more than doubled the transduction rate compared to that without the dilution step. For the DNA fragments used in this study, there was one with a pathogenic origin and two with non-pathogenic origins. However, all three fragments achieved DNA transduction regardless of the difference in their origins. The experimental setup for eliminating the false positives caused by both biological and potentially physical contamination is also described.
此前已有关于DNA在水溶液中进行转导的报道。在本研究中，我们考察了影响极低频电磁场中DNA转导速率的几个关键因素。这些因素包括：水溶液的化学成分、实验容器的类型、稀释步骤以及DNA片段的来源。结果表明，向水溶液中部分引入DNA扩增所需的基本成分（即脱氧核苷三磷酸和PCR缓冲液）可显著提高转导速率，由亲水性石英制成的转导容器比由疏水性塑料制成的容器产生更有利的结果。此外，与不进行稀释步骤相比，对转导溶液进行系列稀释可使转导速率提高一倍以上。本研究中使用的DNA片段，一个来源于病原体，两个来源于非病原体。然而，无论其来源有何差异，所有三个片段均实现了DNA转导。本文还描述了用于消除由生物和潜在物理污染导致的假阳性的实验设置。
