Dunn T L, Seymour G J, Gardiner R A, Strutton G M, Lavin M F
Department of Biochemistry, University of Queensland, St. Lucia, Brisbane.
J Pathol. 1988 Sep;156(1):59-65. doi: 10.1002/path.1711560112.
Activation and/or overexpression of the protein product of the ras gene family (p21ras) has been implicated in the development of various cancers, including bladder carcinoma. We have used the anti-p21ras monoclonal antibody, RAP-5, to assess the level and pattern of expression in formalin-fixed, paraffin-embedded tissue sections of both normal and malignant urothelium. All 14 random normal bladder biopsies and 67 of 68 transitional cell carcinomas of the urinary bladder were positively stained with the RAP-5 antibody. In normal urothelium, p21ras staining tended to be localized to the superficial cell layer. With increasing histological grade and/or depth of invasion of the tumour, a greater proportion of tissue sections demonstrated a staining pattern which was more uniform with respect to the different epithelial cell types. Serially diluting the primary antibody did not reveal any significant differences in the staining patterns observed. Despite the change in staining pattern with increasing grade, these results suggest that p21ras expression by itself is not a useful indicator of the malignant phenotype.
ras基因家族的蛋白产物（p21ras）的激活和/或过表达与包括膀胱癌在内的多种癌症的发生有关。我们使用抗p21ras单克隆抗体RAP-5来评估正常和恶性尿路上皮的福尔马林固定、石蜡包埋组织切片中的表达水平和模式。所有14例随机正常膀胱活检组织以及68例膀胱移行细胞癌中的67例均被RAP-5抗体阳性染色。在正常尿路上皮中，p21ras染色倾向于定位于表层细胞层。随着肿瘤组织学分级的增加和/或浸润深度的增加，更大比例的组织切片显示出一种染色模式，该模式在不同上皮细胞类型方面更加均匀。对一抗进行系列稀释并未发现所观察到的染色模式有任何显著差异。尽管随着分级增加染色模式发生了变化，但这些结果表明p21ras自身表达并非恶性表型的有用指标。
