Gardiner R A, Samaratunga M L, Walsh M D, Seymour G J, Lavin M F
Department of Surgery, University of Queensland, Herston, Australia.
Urol Res. 1992;20(2):117-20. doi: 10.1007/BF00296522.
Sections of formalin-fixed, paraffin-blocked tissue from 116 primary transitional cell carcinomas were stained immunohistochemically using a polyclonal antibody against the c-erbB-2 oncoprotein. Positive staining of cell membranes, known to correlate with gene amplification, was seen in 22 (19%) of the 116, with variable staining from tumour to tumour and within tumours themselves. Consistent with its mooted value as a prognosticator in bladder cancer, the c-erbB-2 oncoprotein was detected in 13 (of 40) grade III and 9 of the 26 muscle-invasive tumours examined compared to 1 (of 25) grade I and 6 (of 66) mucosa only (pTa) lesions. These results support further examination of c-erbB-2 expression in bladder cancer.
使用抗c-erbB-2癌蛋白的多克隆抗体，对116例原发性移行细胞癌的福尔马林固定、石蜡包埋组织切片进行免疫组织化学染色。已知与基因扩增相关的细胞膜阳性染色在116例中有22例（19%）出现，肿瘤之间以及肿瘤内部的染色情况各不相同。与c-erbB-2作为膀胱癌预后指标的推测价值一致，在检查的40例III级肿瘤中有13例、26例肌层浸润性肿瘤中有9例检测到c-erbB-2癌蛋白，相比之下，25例I级肿瘤中有1例、66例仅侵犯黏膜（pTa）的病变中有6例检测到该蛋白（p值）。这些结果支持对膀胱癌中c-erbB-2表达进行进一步研究。
