一种用于培养细胞高分辨率光学显微镜观察的灌注室。

A perfusion chamber for high-resolution light microscopy of cultured cells.

作者信息

Finch S A, Stier A

机构信息

Abteilung Spektroskopie, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, F.R.G.

出版信息

J Microsc. 1988 Jul;151(Pt 1):71-5. doi: 10.1111/j.1365-2818.1988.tb04614.x.

DOI:10.1111/j.1365-2818.1988.tb04614.x

PMID:3057215

Abstract

A perfusion chamber suitable for general microscopic work on upright and inverted microscopes with cultured cells is described. The device features a minimal internal volume and large field of view within small outer dimensions. In particular the chamber body is very flat to accommodate large-aperture condenser and objective lenses. It is constructed of innocuous, sterilizable materials, incorporates permanent seals for the windows, is robust and simple to use.

摘要

描述了一种适用于在正立和倒置显微镜上对培养细胞进行常规显微操作的灌注室。该装置的特点是内部体积最小，在较小的外部尺寸内具有大视野。特别是，腔室主体非常扁平，以适应大孔径聚光镜和物镜。它由无毒、可消毒的材料制成，窗户采用永久性密封，坚固耐用且使用简单。