Lezhnev E I, Lavrovskaia V P, Gavriliuk B K
Tsitologiia. 1977;19(12):1384-6.
The construction of a modified perfusion chamber is presented, which can be used for a prolonged cultivation of mammalian cells and tissues, for observation of the behavior of living cells as well as for the study of different effects on these cells. The chamber is made as non-demountable of optical glass, with a diffusive barrier separating the pericellular zone from that with a perfusion medium. The scheme of the equipment for cultivation of cells and tissues in this diffusion chamber on controlling the composition of nutrient medium and gas phase is given.
本文介绍了一种改良灌注室的构建方法，该灌注室可用于长时间培养哺乳动物细胞和组织，观察活细胞的行为以及研究对这些细胞的不同影响。该室由光学玻璃制成，不可拆卸，带有一个扩散屏障，将细胞周围区域与灌注培养基区域分隔开来。给出了在该扩散室中培养细胞和组织的设备方案，用于控制营养培养基和气相的成分。
