Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing, 210098, PR China; Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, MI, 48824, USA.
Key Laboratory of Integrated Regulation and Resource Development on Shallow Lake of Ministry of Education, College of Environment, Hohai University, Nanjing, 210098, PR China.
Environ Pollut. 2019 Mar;246:357-365. doi: 10.1016/j.envpol.2018.12.030. Epub 2018 Dec 12.
The bacterial communities and ecological contribution of biofilm-leaves of the Vallisneria natans (VN), Hydrilla verticillata (HV) and artificial plant (AP) settled in sediments with different polluted levels of phenanthrene were investigated by high-throughput sequencing in different growth periods. There was no significant difference among the detected Alpha diversity indices based on three classification, attached surface, spiking concentration and incubation time. While Beta diversity analysis assessed by PCoA on operational taxonomic units (OTU) indicated that bacterial community structures were significantly influenced in order of attached surface > incubation time > spiking concentration of phenanthrene in sediment. Moreover, the results of hierarchical dendrograms and heat maps at genus level were consistent with PCoA analysis. We speculated that the weak influence of phenanthrene spiking concentration in sediment might be related to lower concentration and smaller concentration gradient of phenanthrene in leaves. Meanwhile, difference analysis suggested that attached surface was inclined to influence the rare genera up to significant level than incubation time. In general, the results proved that phenanthrene concentrations, submerged macrophytes categories and incubation time did influence the bacterial community of biofilm-leaves. In turn, results also showed a non-negligible ecological contribution of biofilm-leaves in dissipating the phenanthrene in sediments (>13.2%-17.1%) in contrast with rhizosphere remediation (2.5%-3.2% for HV and 9.9%-10.6% for VN).
采用高通量测序技术，研究了不同污染水平菲在沉水植物菹草（VN）、黑藻（HV）和人工植物（AP）叶片生物膜中的细菌群落组成及其生态贡献。在三种分类（附着面、投加浓度和培养时间）下，基于检测到的Alpha 多样性指数没有显著差异。然而，基于操作分类单元（OTU）的 PCoA 分析表明，细菌群落结构受到显著影响，其顺序为附着面>培养时间>沉水植物叶片中菲的投加浓度。此外，属水平的层次聚类和热图结果与 PCoA 分析一致。我们推测，沉水植物叶片中菲投加浓度的微弱影响可能与叶片中菲的浓度较低且浓度梯度较小有关。同时，差异分析表明，与培养时间相比，附着面更倾向于显著影响稀有属。总的来说，结果证明了菲浓度、沉水植物种类和培养时间确实影响了生物膜叶片中的细菌群落。反过来，结果还表明，生物膜叶片在消耗沉积物中菲方面具有不可忽视的生态贡献（>13.2%-17.1%），与根际修复（HV 为 2.5%-3.2%，VN 为 9.9%-10.6%）相比。
