Ministry of Education Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, College of Environment, Hohai University, Nanjing, 210098, China.
Sci Rep. 2018 Feb 8;8(1):2650. doi: 10.1038/s41598-018-21080-y.
The effects of water flow on the leaf-biofilm interface of Vallisneria natans and Hydrilla verticillata were investigated using artificial plants as the control. Water flow inhibited the growth of two species of submerged macrophytes, reduced oxygen concentrations in plant leaves and changed oxygen profiles at the leaf-biofilm interface. The results from confocal laser scanning microscopy and multifractal analysis showed that water flow reduced biofilm thickness, changed biofilm topographic characterization and increased the percentages of single colony-like biofilm patches. A cluster analysis revealed that the bacterial compositions in biofilms were determined mainly by substrate types and were different from those in sediments. However, water flow increased the bacterial diversity in biofilms in terms of operational taxonomic unit numbers and Shannon Indices. Our results indicated that water flow can be used to regulate the biomass, distribution and bacterial diversities of epiphytic biofilms in constructed wetlands dominated by submerged macrophytes.
采用人工植物作为对照，研究了水流对苦草和黑藻叶-生物膜界面的影响。水流抑制了两种沉水植物的生长，降低了植物叶片中的氧浓度，并改变了叶-生物膜界面处的氧分布。共焦激光扫描显微镜和多重分形分析的结果表明，水流减少了生物膜的厚度，改变了生物膜的地形特征，并增加了单菌落样生物膜斑块的比例。聚类分析表明，生物膜中的细菌组成主要由基质类型决定，与沉积物中的细菌组成不同。然而，水流增加了生物膜中细菌的多样性，表现在操作分类单元数量和 Shannon 指数上。我们的结果表明，水流可以用来调节以沉水植物为主的人工湿地中附生生物膜的生物量、分布和细菌多样性。
