Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Chennai campus, Vandalur-Kelambakkam Road, Chennai 600 127, Tamil Nadu, India.
Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632 014, Tamil Nadu, India.
Mater Sci Eng C Mater Biol Appl. 2019 Feb 1;95:312-327. doi: 10.1016/j.msec.2018.11.002. Epub 2018 Nov 5.
Utilization of protein-protein interactions or protein-peptide interactions has led to new crosslinking chemistries, resulting into protein hydrogels. Enzyme catalyzed crosslinking of specific amino acids has also been used to generate crosslinked protein hydrogels. Weak, temporary, reversible or non-covalently crosslinked protein gels as well as strong, permanent, irreversible or covalently crosslinked protein gels with mechanical strengths of varying degrees are generated by means of various crosslinking strategies. These protein hydrogels are tailored by means of protein engineering and recombinant DNA technology, depending on its end use as scaffolds for specific tissue engineering, drug delivery, wound dressings etc. This review aims to cover the advancements in the use of protein engineering along with different crosslinking techniques to create novel protein hydrogels that finds various applications in biomedical industries.
利用蛋白质-蛋白质相互作用或蛋白质-肽相互作用已经产生了新的交联化学物质，从而形成蛋白质水凝胶。酶催化特定氨基酸的交联也被用于生成交联的蛋白质水凝胶。通过各种交联策略，可以生成具有不同机械强度的弱、临时、可逆或非共价交联的蛋白质凝胶以及强、永久、不可逆或共价交联的蛋白质凝胶。这些蛋白质水凝胶可以通过蛋白质工程和重组 DNA 技术进行定制，具体取决于其最终用途，例如作为特定组织工程、药物输送、伤口敷料等的支架。本综述旨在涵盖利用蛋白质工程和不同交联技术来创建新型蛋白质水凝胶的进展，这些水凝胶在生物医学行业中有多种应用。
