Univ. Limoges, CNRS, IRCER, UMR 7315, F-87000 Limoges, France.
Ecole Nationale Supérieure des Mines, CIS-EMSE, INSERM U1059, 158 cours Fauriel, F-42023 Saint-Etienne cedex 2, France.
Mater Sci Eng C Mater Biol Appl. 2019 Feb 1;95:343-354. doi: 10.1016/j.msec.2018.01.008. Epub 2018 Jan 31.
This work is devoted to the processing of bone morphogenetic protein (BMP-2) functionalized silicate substituted hydroxyapatite (SiHA) ceramic spheres. The motivation behind it is to develop injectable hydrogel/bioceramic composites for bone reconstruction applications. SiHA microspheres were shaped by spray drying and thoroughly characterized. The silicate substitution was used to provide preferred chemical sites at the ceramic surface for the covalent immobilization of BMP-2. In order to control the density and the release of the immobilized BMP-2, its grafting was performed via ethoxysilanes and polyethylene glycols. A method based on Kaiser's test was used to quantify the free amino groups of grafted organosilanes available at the ceramic surface for BMP-2 immobilization. The SiHA surface modification was investigated by means of X-ray photoelectron spectroscopy, Fourier transformed infrared spectroscopy and thermogravimetry coupled with mass spectrometry. The BMP-2 bioactivity was assessed, in vitro, by measuring the luciferase expression of a stably transfected C3H10 cell line (C3H10-BRE/Luc cells). The results provided evidence that the BMP-2 grafted onto SiHA spheres remained bioactive.
这项工作致力于处理骨形态发生蛋白（BMP-2）功能化的硅酸取代羟基磷灰石（SiHA）陶瓷球。其背后的动机是开发用于骨重建应用的可注射水凝胶/生物陶瓷复合材料。通过喷雾干燥成型并对 SiHA 微球进行了全面表征。硅酸根取代用于在陶瓷表面提供优先的化学位，以便通过共价键固定 BMP-2。为了控制固定 BMP-2 的密度和释放，通过乙氧基硅烷和聚乙二醇进行了其接枝。使用基于 Kaiser 测试的方法来定量陶瓷表面上用于 BMP-2 固定的接枝有机硅烷的游离氨基。通过 X 射线光电子能谱、傅里叶变换红外光谱和热重分析与质谱联用研究了 SiHA 表面改性。通过测量稳定转染的 C3H10 细胞系（C3H10-BRE/Luc 细胞）的荧光素酶表达，体外评估了 BMP-2 的生物活性。结果表明，接枝到 SiHA 球上的 BMP-2 仍具有生物活性。
