ICMN, CNRS, Université d'Orléans, UMR 7374, F-45071 Orléans, France.
iBV, CNRS, INSERM, Université Côte d'Azur, F-06107 Nice, France.
Int J Mol Sci. 2021 Nov 12;22(22):12247. doi: 10.3390/ijms222212247.
A biomaterial that is both bioactive and capable of controlled drug release is highly attractive for bone regeneration. In previous works, we demonstrated the possibility of combining activated carbon fiber cloth (ACC) and biomimetic apatite (such as calcium-deficient hydroxyapatite (CDA)) to develop an efficient material for bone regeneration. The aim to use the adsorption properties of an activated carbon/biomimetic apatite composite to synthetize a biomaterial to be used as a controlled drug release system after implantation. The adsorption and desorption of tetracycline and aspirin were first investigated in the ACC and CDA components and then on ACC/CDA composite. The results showed that drug adsorption and release are dependent on the adsorbent material and the drug polarity/hydrophilicity, leading to two distinct modes of drug adsorption and release. Consequently, a double adsorption approach was successfully performed, leading to a multifunctional and innovative ACC-aspirin/CDA-tetracycline implantable biomaterial. In a second step, in vitro tests emphasized a better affinity of the drug (tetracycline or aspirin)-loaded ACC/CDA materials towards human primary osteoblast viability and proliferation. Then, in vivo experiments on a large cortical bone defect in rats was carried out to test biocompatibility and bone regeneration ability. Data clearly highlighted a significant acceleration of bone reconstruction in the presence of the ACC/CDA patch. The ability of the aspirin-loaded ACC/CDA material to release the drug in situ for improving bone healing was also underlined, as a proof of concept. This work highlights the possibility of bone patches with controlled (multi)drug release features being used for bone tissue repair.
一种既具有生物活性又能够控制药物释放的生物材料对于骨再生极具吸引力。在之前的工作中，我们证明了将活性碳纤维布（ACC）和仿生磷灰石（如缺钙羟基磷灰石（CDA））结合起来开发用于骨再生的有效材料的可能性。目的是利用活性炭/仿生磷灰石复合材料的吸附性能，合成一种可作为植入物后控制药物释放系统的生物材料。首先研究了四环素和阿司匹林在 ACC 和 CDA 成分中的吸附和解吸，然后研究了在 ACC/CDA 复合材料中的吸附和解吸。结果表明，药物吸附和解吸取决于吸附剂材料和药物的极性/亲水性，导致药物吸附和解吸的两种不同模式。因此，成功地采用了双重吸附方法，制备了一种多功能、创新的 ACC-阿司匹林/CDA-四环素植入式生物材料。在第二步，体外试验强调了载药（四环素或阿司匹林）的 ACC/CDA 材料对人原代成骨细胞活力和增殖的更好亲和力。然后，在大鼠大皮质骨缺损的体内实验中测试了生物相容性和骨再生能力。数据清楚地表明，在存在 ACC/CDA 补片的情况下，骨重建明显加快。载阿司匹林的 ACC/CDA 材料原位释放药物以改善骨愈合的能力也得到了强调，这是一个概念验证。这项工作强调了具有控制（多）药物释放特性的骨贴剂用于骨组织修复的可能性。
