Bancerz-Kisiel Agata, Gral Magdalena, Odyniec Marta, Szweda Wojciech
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719, Olsztyn, Poland.
Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 2, 10-719, Olsztyn, Poland.
Comp Immunol Microbiol Infect Dis. 2019 Apr;63:131-135. doi: 10.1016/j.cimid.2019.01.020. Epub 2019 Feb 2.
The aim of the study was to determine whether the presence of the Yersinia virulence plasmid could affect the production of enterotoxin YstA by Y. enterocolitica strains isolated from pigs which are the main source of infection for humans. The phenotypic features characteristic for the Yersinia virulence plasmid were detected on CRMOX agar in 8 out of 12 strains producing enterotoxin YstA, in 5 out of 12 doubtful strains, and in 11 out of 12 strains not producing YstA. Autoagglutination ability was detected in all 12 Y. enterocolitica strains that were positive in the suckling mice bioassay, in 11 doubtful strains and 10 negative strains. CRMOX+ colonies were generally ystA, myfA, virF and yadA positive, while CRMOX- colonies were only ystA and myfA positive. The amplicons of yadA were not detected in 2 (8.3%) out of 24 CRMOX+ and virF positive strains. The results of this study indicate that the presence of pYV does not affect the enterotoxin-producing ability of Y. enterocolitica strains.
本研究的目的是确定耶尔森氏菌毒力质粒的存在是否会影响从猪中分离出的小肠结肠炎耶尔森氏菌菌株产生肠毒素YstA的能力，猪是人类主要的感染源。在12株产生肠毒素YstA的菌株中，有8株在CRMOX琼脂上检测到了耶尔森氏菌毒力质粒的表型特征；在12株可疑菌株中，有5株检测到；在12株不产生YstA的菌株中，有11株检测到。在所有12株在乳鼠生物测定中呈阳性的小肠结肠炎耶尔森氏菌菌株、11株可疑菌株和10株阴性菌株中均检测到自凝能力。CRMOX+菌落通常ystA、myfA、virF和yadA呈阳性，而CRMOX-菌落仅ystA和myfA呈阳性。在24株CRMOX+且virF阳性的菌株中，有2株（8.3%）未检测到yadA的扩增子。本研究结果表明，pYV的存在不会影响小肠结肠炎耶尔森氏菌菌株产生肠毒素的能力。
